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Test Code 167 Wound Culture w/ gram stain

Epic Code


Test Name Alias

Culture Wound w/gram | Serratia screen | 167

Specimen Requirements

Submit only one of the following specimens


Specimen Type: Varied Swab (sites vary: state source of specimen)
Container/Tube: eSwab
Collection Instructions:

  1. Cleanse skin or mucosal area
  2. Open wounds, debride and rinse w/sterile saline
  3. Sample viable infected tissue, not superficial debris.

How to use eSwab:

  1. Open the peel pouch and remove the contents. Aseptically unscrew and remove the cap from the eSwab.

  2. Place the tube in a secure position so as not to allow the liquid to leak out of the tube.

  3. Remove the swab. Collect the patient's sample using the swab. (If vaginal/rectal swab, collect a specimen from the vaginal area and then collect from the rectal area.)

  4. Insert the swab back into the tube of liquid, all the way to the bottom of the tube.

  5. Holding the swab shaft close to the rim of the tube, break the applicator shaft at the color breakpoint indication line.

  6. Screw the cap on tightly to prevent leakage. Discard the broken handle of the shaft into an approved medical waste disposal container.

  7. Write the patient's information (Name and Date of Birth) on the tube or apply a label with patient’s information. Indicate source.

  8. Send specimen to the Laboratory.

Opthamology Services (Eye Profile):

Specimen Type: Corneal scrapings

Container/Tube: 1 Blood agar plate, 1 Chocolate agar plate, 1 air-dried slide for gram stain

Collection Instructions:   Order bacterial, fungal, and AFB test components separately.  Please state on the requisition that the sample is for eye profile protocol.


Send aspirates for abcesses, see body fluid culture.

Special Instructions

The ESwab fluid maintains the viability of diverse bacteria. Do not send a dry ESwab as this will lead to unsatisfactory results. If the tube spills its contents prior to inserting the swab, the liquid is non-toxic. Simply put the swab into another tube before sending it to the laboratory and discard the spilled tube.

Specimen Stability

Ambient: 48 hours

Refrigerate: 48 hours

Laboratory Retention: 7 days

Test Frequency

Available daily, usual TAT 18 – 24 hours for preliminary results, 3 days for final results.

Reference Range

Bacteria not isolated

Performing Department


Performing Department Laboratory Location

Spectrum Health Regional Laboratory (SHRL), Grand Rapids, MI
Spectrum Health Gerber Laboratory (SHGH), Fremont, MI
Spectrum Health Reed City Laboratory (SHRC), Reed City, MI





If identification and/or susceptibility are indicated additional charges may follow

CDM Code


Interface Order Alias


Mayo Access Code


Additional Information

Aerobic swab – Red top with sponge in bottom may be an acceptable container until eswabs are available.

Wound Culture should be ordered for inpatient Serratia screening when indicated. Please write "Serratia Screen" in order comments.

Reviewed Date