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Epic Code LAB1230913 Alkaline Phosphatase Isoenzymes

Additional Codes

Beaumont Code: LAB5846

Interface Order Alias

1230913

Clinical Information

This assay is used to differentiate between liver, bone and intestinal isoenzymes in patients with an elevated serum alkaline phosphatase.

Collection Instructions

Container(s): Gold top (Serum Separator SST) 

Preferred Volume to Collect: 5 mL

Minimum Volume to Collect: 2 mL

Submission Container/Tube:  Plastic vial

Collection Instructions:

  • After collection, gently invert tube 8-10 times.
  • Specimen should be processed within 2 hours – See Processing Instructions below.

 

 

PROCESSING INSTRUCTIONS (Laboratory, Outpatient or Off-site collection)

Processed Specimen: Serum

Centrifuge/Spin: Yes

Aliquot: Yes

Processing Instructions:

  • Allow blood to clot for 30 minutes in a vertical position and centrifuge promptly.
  • Within two hours aliquot serum into a plastic transport tube and refrigerate as soon as possible.
  • (+(2)S/PO/R)

Specimen Transport Temperature

Refrigerated

Rejection Criteria

  • Plasma specimens. 
  • Grossly hemolyzed, lipemic or icteric specimens. 
  • Red-top tubes with serum not separated from cells within two hours of collection.
  • Specimens with total Alkaline Phosphatase levels lower than 111 U/L.

Specimen Stability

Serum Specimens (Pour-Overs)

Ambient: 2 hours

Refrigerated: 7 days

Test Frequency

3 times a week, afternoons.
Routine results available within 7 days.

Reference Range

Adult male & female:

Total Alkaline Phosphatase: 30-110 U/L.
Total Liver Fraction (Includes Liver 2): 25-74 U/L.
Liver 2 Fraction: 2-17 U/L.
Bone Fraction: 9-44 U/L.
Intestinal Fraction: 1-11 U/L.

Performing Department

REFERRAL SEND OUTS

Performing Department Laboratory Location

Beaumont Royal Oak Special Testing Laboratory

Methodology

Separation by Agarose Gel Electrophoresis.

CPT

84075, 84080

CDM Code

3018407503, 3018408005

Epic Test ID

1230102583

LOINC

6768-6

Reviewed Date

6/9/2023