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Epic Code LAB2111011 BCR-ABL1 t[9;22] RT- PCR

Test Name Alias

BCR-ABL1 t[9 |22] RT-PCR | 9011

Interface Order Alias

11111

Quick Collect

Blood, BM = +A

Clinical Information

The BCR-ABL1 assay uses real-time rtPCR to detect the BCR-ABL1 major (p210) and minor (p190) transcripts from the t(9;22) translocation, which can be used to monitor minimal residual disease in patients with CML.

Collection Instructions

Multiple specimen collection types. Submit only one:

 

Specimen Collection: Blood OR Bone Marrow (BM)


Container/Tube: 6.0 mL Pink EDTA or Lavender EDTA

Preferred volume to collect: 6.0 mL

Minimum volume to collect: 6.0 mL

 

Collection Instructions:

  • Gently invert tube 8-10 times after collection
  • For optimal results, specimen should be received and processed within 24 hours of collection.

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Spin: No

Aliquot: No

Transport Temperature: If received within 24 hours of collection, specimen can be transported at ambient temperature or refrigerated.

If transport will exceed 24 hours, recommend specimen be refrigerated.

Specimen Stability

Ambient: <24 hours

Refrigerate: <72 hours

Laboratory Retention: Extracted RNA retained greater than 1 year

Test Frequency

Available at least once per week, usual TAT less than 7 days

Reference Range

An interpretative report will be provided.

Performing Department

Molecular Diagnostics

Performing Department Laboratory Location

Corewell Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Real-Time Quantitative Polymerase Chain Rection (RT-qPCR)

CPT

81207, 81206

CDM Code

3108120701, 3108120601

Epic Test ID

1230100376

LOINC

Specimen Source: 31208-2

Specimen ID: 8251-1

BCR-ABL t[9:22]: 63422-0

BCR-ABL t[9:22] RT-PCR Ratio: 69380-4

BCR-ABL Log Reduction: 50195-7

BCR-ABL comm: 8251-1

Mayo Access Code

SHO9011

Reviewed Date

6/22/2023