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Epic Code LAB2111373 Cytogenetics Fibroblast Culture for Reference Testing with Cryopreservation

Test Name Alias

Cytogenetics Fibroblast Culture for Reference Testing with Cryopreservation | 930

Interface Order Alias

11387

Quick Collect

AP Ambient

Collection Instructions

Multiple specimen collection types. Submit only one:

 

Specimen Collection: Amniotic fluid

Container(s): 2 Sterile screw-capped conical tubes

Preferred volume to collect: 30.0 mL

Minimum volume to collect: 20.0 mL

Collection Instructions:

  • Discard first 2 mL to prevent contamination with maternal cells.

 

Specimen Collection: Skin biopsy

Container(s): Sterile transport tube with tissue culture media

Preferred volume to collect: 5.5 mm

Minimum volume to collect: 3.0 mm

Collection Instructions:

  • Place in sterile container with either tissue culture media or transport media and an antibiotic such as Pen/Strep or Gentamicin.
  • Tissue may be stored overnight in medium at 4°C.
  • Specimens placed in formalin are not acceptable.

 

 

Specimen Collection: Surgery/Autopsy biopsy or Products of Conception

Container(s): Sterile transport tube with tissue culture media

Preferred volume to collect: 1 cm3

Minimum volume to collect:

Collection Instructions:

  • Tissue may be stored overnight in medium at 4°C.
  • Specimens placed in formalin are not acceptable.

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Spin: No

Aliquot: No

 

Transport Temperature: Ambient (Tissue may be stored overnight in medium at 4°C.

 

If ambient temperature exceeds 80°F or is below 40°F, please send in an insulated container. All samples should be directed to the laboratory as soon as possible.

 

Coordinate request for reference testing directly with Referrals Department to determine ordering requirements. Please call 616.267.2753.

 

Please provide all necessary documentation for the desired reference testing with the specimen to ensure that the cultures are prepared and sent according to the reference laboratory’s specifications.

Specimen Stability

Ambient:  3 days (beyond 3 days, specimen viability will be determined by Cytogenetics Lab.)

Laboratory Retention: The cultures will be cryopreserved to ensure the specimen is not lost/damaged in transit and for potential required additional testing.

Test Frequency

Test Set Up: Monday – Friday 8:00 am – 5:00 pm, Saturday 8:00 am – 3:00 pm.

Technologist on call for Sunday set up.

Reference Range

Confirmation that fibroblast cultures have been forwarded to the Referrals department for the desired reference testing will be provided.

Performing Department

Cytogenetics

Performing Department Laboratory Location

Corewell Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Fibroblasts are cultured and prepared according to the reference lab's requirements. Additional fibroblast cultures are suspended in 10% Dimethylsulfoxide (DMSO) freeze media for prolonged storage of viable cells in liquid nitrogen.

CPT

88233, 88240

CDM Code

3108823301, 3108824001

Epic Test ID

1230100589

Reviewed Date

4/28/2023