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Epic Code LAB2111441 Cytogenetics Fibroblast Culture with Cryopreservation

Test Name Alias

Cytogenetics Fibroblast Culture with Cryopreservation | 929

Interface Order Alias

11455

Quick Collect

AP Ambient

Collection Instructions

Multiple specimen collection types. Submit only one:

 

Specimen Collection: Amniotic fluid

Container(s): 2 Sterile screw-capped conical tubes

Preferred volume to collect: 30.0 mL

Minimum volume to collect: 20.0 mL

Collection Instructions:

  • Discard first 2 mL to prevent contamination with maternal cells.

 

Specimen Collection: Skin biopsy

Container(s): Sterile transport tube with tissue culture media

Preferred volume to collect: 5.5 mm

Minimum volume to collect: 3.0 mm

Collection Instructions:

  • Place in sterile container with either tissue culture media or transport media and an antibiotic such as Pen/Strep or Gentamicin.
  • Tissue may be stored overnight in medium at 4°C.
  • Specimens placed in formalin are not acceptable.

 

 

Specimen Collection: Surgery/Autopsy biopsy or Products of Conception

Container(s): Sterile transport tube with tissue culture media

Preferred volume to collect: 1 cm3

Minimum volume to collect:

Collection Instructions:

  • Tissue may be stored overnight in medium at 4°C.
  • Specimens placed in formalin are not acceptable.

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Spin: No

Aliquot: No

 

Transport Temperature: Ambient (Tissue may be stored overnight in medium at 4°C)

If ambient temperature exceeds 80°F or is below 40°F, please send in an insulated container. All samples should be directed to the laboratory as soon as possible.

 

Specimens that have been placed in formalin are not acceptable.

Specimen Stability

Ambient: 3 days (beyond 3 days, specimen viability will be detemined by Cytogenetics Lab)

Laboratory Retention: primary specimens 4 weeks; frozen pellets retained greater than 1 year

Test Frequency

Test Set Up: Monday – Friday 8:00 am – 5:00 pm, Saturday 8:00 am – 3:00 pm.

Technologist on call for Sunday set up.

Reference Range

Confirmation that fibroblasts cultures have been cryopreserved for potential future testing will be provided.

Performing Department

Cytogenetics

Performing Department Laboratory Location

Corewell Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Fibroblasts are cultured and suspended in 10% Dimethylsulfoxide (DMSO) freeze media for prolonged storage of viable cells in liquid nitrogen.

CPT

88233, 88240

CDM Code

3108823301, 3108824001

Epic Test ID

1230100590

Reviewed Date

4/28/2023