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Epic Code LAB3429 BRAF Mutation Analysis

Test Name Alias

BRAF Mutation Analysis by Next Generation Sequencing | 11499 | 4155 | LAB2111583 | 709

Interface Order Alias

11723

Quick Collect

Tissue, Other = LOOK+LOOK Paraffin Block = Mark Tumor and Normal

Collection Instructions

Multiple specimen collection types. Submit only one:

 

Specimen Type: Formalin-fixed, paraffin-embedded (FFPE) tissue (block or 10 unstained slides) accompanied by an H&E slide scribed by Pathologist and Surgical Specimen Testing order sheet.

Specimen Collection: Place specimen in 10% Formalin and order Tissue Pathology in addition to BRAF Mutation Analysis order.

Transport Temperature: Ambient

  

Specimen Type: Fresh tissue, flash frozen, then shipped on dry ice.

 

Specimen Type: Whole Blood EDTA

Container/Tube: Laveder Top (EDTA)

Volume: 3.0 mL

Minimum volume: 1.0 mL

Neonate volume: 1.0 mL (2 microtainers acceptable)

Okay for Capillary Collect: Yes

Transport Temperature: Ambient

 

Specimen Type: Bone marrow EDTA

Container/Tube: Lavender Top (EDTA)

Volume: 3.0 mL

Minimum volume: 1.0 mL

Transport Temperature: Ambient

Specimen Stability

Laboratory Retention: Extracted DNA retained greater than 1 year

Reflex Information

MEC approved

Mandatory Reflex Information:

  • Metastic melanoma, BRAF will be performed

Test Frequency

Available once per week, usual TAT less than 7 days.

Reference Range

An interpretative report will be provided.

Performing Department

Molecular Diagnostics

Performing Department Laboratory Location

Corewell Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Next generation sequencing by Ion AmpliSeq technology

CPT

81210

CDM Code

3108121001

Epic Test ID

1230100409

LOINC

Specimen ID:  8251-1

Tissue Source:  31208-2

Viable Tumor Cells %:  8251-1

BRAF Mutation:  53844-7

BRAF Comment:  8251-1

Reviewed Date

6/22/2023