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Test Code LAB119 Protein Electrophoresis, Serum

Test Name Alias

Protein Electrophoresis Serum | SPEP | S-PEP | 8305

Clinical Information

This test will include serum protein and pathologist interpretation.

Collection Instructions

Specimen Collection: Blood

Container(s): Gold Top (Serum Separator-SST Gel)

Preferred Volume to Collect: 5.0 mL

Minimum Volume to Collect: 2.0 mL

Neonate Volume to Collect: 2.0 mL

Capillary collect ok: Yes

Microtainer acceptable: Yes

Collection Instructions:

  • Fasting specimen preferred.
  • Gently invert gold tube(s) 8 - 10 times to mix clot activator with blood.
  • Specimen should be processed within 2 hours – See Processing Instructions.
  • Protein Electrophoresis cannot be ordered a second time within a 2 week window in Laboratory Information System (LIS)

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Processed Specimen: Serum

Centrifuge/Spin: Yes

Aliquot: No

Processing Instructions:

  • Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.

Transport Temperature: Refrigerate

Rejection Criteria

Specimen not centrifuged within 2 hours.

Reflex Information

Pathologist will review serum electrophoresis and if necessary a serum immunotyping (immunofixation) will be performed at an additional charge.

Specimen Stability

Ambient: 8 hours

Refrigerate: 7 days

Frozen: aliquot serum for longer storage

Laboratory Retention: 7 days

Test Frequency

Available Monday – Friday, routine TAT 5 days.

Reference Range

Albumin SPE
3.7 – 4.9 g/dL

 

Alpha 1 Glob
0.2 – 0.4 g/dL

 

Alpha 2 Glob
0.5 – 0.9 g/dL

 

Beta Glob
0.6 – 1.0 g/dL

 

Gamma Glob
0.6 – 1.4 g/dL

 

Total Protein
0 Minutes – 8 Days: 4.5 – 7 g/dL
8 Days – 3 Years: 5.5 – 7.5 g/dL
3 Years – 150 Years: 6 – 8 g/dL

Performing Department

IMMUNOCHEMISTRY

Performing Department Laboratory Location

Spectrum Health Regional Laboratory, Grand Rapids, MI

Methodology

Capillary Zone Electrophoresis

CPT

84165

Professional fee for Pathologist interpretation

CDM Code

4058305

Epic Test ID

1230101085

Interface Order Alias

10232

Mayo Access Code

SHO138

Reviewed Date

7/17/2017