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Epic Code LAB1230279 Protein Electrophoresis, CSF and Serum

Important Note

Note: 2 specimen types - both specimens should be drawn and received together; however, serum collected within 72 hours of the CSF collection will be acceptable.

Test Name Alias

Protein Electrophoresis CSF | Oligoclonal bands CSF | 8651

Interface Order Alias

11602

Clinical Information

Reported with Pathologist interpretation. 

Panel Information

IgG Index, Serum

IgG Index Synth, CSF

Protein Electrophoresis, CSF and Serum

Collection Instructions

Submit 2 specimen types: CSF and Blood

 

Specimen Collection: Spinal Fluid (CSF)

Container(s): Sterile transport tube

Preferred Volume to Collect:

Minimum Volume to Collect: 1.0 mL

Neonate Volume to Collect: 1.0 mL

Collection Instructions: Label tube as spinal fluid (along with 2 patient identifiers)

 

Specimen Collection: Blood

Container(s): Gold Top (Serum Separator-SST Gel)

Preferred Volume to Collect: 5.0 mL

Minimum Volume to Collect: 2.0 mL

Neonate Volume to Collect: 2.0 mL

Capillary collect ok? Y

Microtainer acceptable: Y

Collection Instructions:

  • Gently invert gold tube(s) 5 times to mix clot activator with blood.
  • Blood may be collected within 72 of CSF collection.
  • Blood should be processed within 2 hours – See Processing Instructions.

2 specimen types must be submitted

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Submit 2 specimen types: CSF and Blood

 

CSF

No processing instructions

 

Blood 

Processed Specimen: Serum

 

Centrifuge/Spin: Y

Aliquot: N

 

Processing Instructions: Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.

 

Transport Temperature: Refrigerate

Lab Staff Instructions

Lab Central Staff: All CSF specimens to Hematology first.

Rejection Criteria

Specimen not centrifuged within 2 hours.
Serum not collected within 72 hours of CSF collection.

Specimen Stability

SERUM:     

Ambient: 8 hours

Refrigerate: 7 days

Frozen: 1 month

Laboratory Retention: 1 month

 

CSF:

Refrigerate: 7 days

Frozen: 1 month

Test Frequency

Test run once per week on Thursdays.

Reference Range

Negative

No bands present in either serum or CSF or one unique band in the CSF compared to the serum

Equivocal

2-3 unique bands in the CSF compared to the serum

Positive

≥4 unique bands in the CSF compared to the serum

 

IgG, Serum: 700-1,500 mg/dL

IgG, CSF: 0.5-5.9 mg/dL

Performing Department

Multiple Departments: Chemistry and Immunochemistry

Performing Department Laboratory Location

Corewell Health Reference Laboratory, Grand Rapids, MI

Methodology

Isoelectricfocusing followed with immunofixation using anti-IgG antiserum

CPT

82040, 82784, 82042, 82784, 84157, 83916
Professional fee for pathologist interpretation

CDM Code

3018204201, 3018278403, 3018415701, 3018204201,3018278403, 3018391601

Epic Test ID

1230100138, 1230100139, 1230100183

Mayo Access Code

SHO8651

Reviewed Date

3/23/2022