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Epic Code LAB1230328 B-ALL MRD, Bone Marrow

Test Name Alias

B Lymphoblastic Leukemia MRD | B cell acute leukemia MRD | Minimal residual disease | MRD by Flow Cytometry | COG Day 29 | B-ALL MRD

Interface Order Alias

55132

Clinical Information

This test is appropriate for patients previously diagnosed wth B-ALL and  may be run at any time point in therapy.

 

This is a COG approved MRD test for patients enrolled on AALL0932 and AALL1131. Day 29 of COG protocol. May be ordered post consolidation; other timepoints in therapy also accepted.

 

Minimal residual disease (MRD) is a powerful prognostic factor for predicting outcome in pediatric B cell acute lymphoblastic leukemia (B-ALL).  This flow cytometry assay is an effective method for measuring MRD and is the COG approved MRD test for patients enrolled on AALL0932 and AALL1131 Day 8 of COG protocol; other time points in therapy also accepted.  This flow cytometry assay involves the staining of white blood cells in a bone marrow sample with a viability dye and monoclonal antibody to CD3, CD9, CD10, CD13, CD19, CD20, CD33, CD34, CD38, CD45, CD58, and CD71. Additional markers may be evaluated if phenotypic charateristics indicate the need. This flow cytometric assay has the ability to enumerate and characterize very small populations of abnormal cells, often as low as 1 in 10,000 cells.  

Collection Instructions

Specimen Collection: Bone Marrow Aspirate


Container/Tube: 4 mL Sodium Heparin (NaHep Green) or 3 mL Lavender EDTA

Preferred volume to collect: 4.0 mL NaHep or 3.0 mL EDTA

Minimum volume to collect: 3.0 mL

Neonate volume to collect: 2.0 mL

 

Collection Instructions:

  • Keep sample at room temperature and send to the Lab immediately. 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Please contact ATL Lab Central at 616-486-6233 for shipping instructions

 

Spin: No

Aliquot: No

 

Transport Temperature: Ambient

 

If ambient temperature exceeds 80°F or is below 40°F, please send in an insulated container. All samples should be directed to the laboratory as soon as possible.

Specimen Stability

Ambient: 48 hours (specimens >48 hours will still be run and reported)

Laboratory Retention: 7 days (refrigerated)

Test Frequency

Test Set Up: Monday – Friday 8:00 am – 4:00 pm, Saturday - Sunday 8:00 am - 12:30 pm

 

Final report: 1 – 3 days.

Reference Range

An interpretative report will be provided.

Performing Department

FLOW CYTOMETRY

Performing Department Laboratory Location

Spectrum Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Flow Cytometry Immunophenotyping

CPT

88184

88185x12

88188

Epic Test ID

1230101893

Beaker LOINC

LOINC Not available

Reviewed Date

7/31/2021

Beaker Names

Beaker Procedure Name: B-ALL MRD BONE MARROW BY FLOW CYTOMETRY
Beaker Display Name: B-ALL MRD BONE MARROW by Flow Cytometry
BEAKER TEST NAME: B-ALL MRD BONE MARROW BY FLOW CYTOMETRY
BEAKER TEST REPORT NAME: B-ALL MRD Bone Marrow by Flow Cytometry

Beaker Synonyms

No synonym on file

Beaker Collection and Specimen Handling

BEAKER COLLECTION: AP Ambient

Beaker Location, Container and Temperature

LC FLOW CYTOMETRY: 4 ML GREEN NA HEPARIN TUBE (Preferred)-Ambient
LC FLOW CYTOMETRY: 3 ML LAVENDER TOP TUBE A (Acceptable)-Ambient