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Test Code LAB1230390 Immature Platelet Fraction (Reflex Only, Lab Only Orderable)

Test Name Alias

Reticulated platelets | immature platelets | young platelets | IPF

Clinical Information

Immature platelet fraction (IPF) is a direct indicator of bone marrow thrombopoietic activity that may aid clinicians in the evaluation of thrombocytopenia. The IPF is useful in determining whether the thrombocytopenia is secondary to decreased production or peripheral destruction of the platelets. Different studies showed that patients with peripheral platelet destruction (such as ITP, TTP) have significantly higher IPF than those with marrow suppression.

IPF is a good predictor of platelet recovery and can be used to monitor the patients rebounding from hematopoietic progenitor cell (HPC) transplantation or chemotherapy.


Reference: Kickler TS, Oguni S, Borowitz MJ. Clinical evaluation of high fluorescent platelet fraction percentage in thrombocytopenia. Am J Clin Pathology 2006;125:282-287.

Ordering Instructions

This test cannot be ordered separately, it is only available as a reflex test.

Collection Instructions

Specimen Collection: Blood


Container(s): Lavender top (EDTA)

Preferred Volume to Collect: 3.0 mL

Minimum Volume to Collect: 0.5 mL

Neonate Volume to Collect: 0.5 mL

Capillary collect ok? Yes

Microtainer acceptable: Yes


Collection Instructions:

  • Gently invert EDTA tube(s) 7 – 8 times immediately after collection.

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Processed Specimen: Whole Blood EDTA

Centrifuge/Spin: No

Aliquot: No


Transport Temperature: Room Temperature

Rejection Criteria

Age of specimen

Specimen Stability

Ambient: 24 hours

Refrigerate: 24 hours

Frozen: Not acceptable

Laboratory Retention: 72 hours

Reflex Information

This test will reflex on a platelet count <75,000 on a CBC w/Diff, CBC w/Manual Diff, or CBC w/o Diff, and Platelet count. Platelet Citrate will not reflex.

Test Frequency

Available daily, usual TAT 1 day.

Reference Range

Immature Platelet Fraction (IPF %)

0.9 - 8.5%

Immature Platelet Fraction (IPF) Absolute

3.4 - 18.4 x10^3/uL


Performing Department


Performing Department Laboratory Location

Spectrum Health Regional Laboratory, Grand Rapids, MI

Spectrum Health Big Rapids Laboratory, Big Rapids, MI

Spectrum Health Blodgett Laboratory, Grand Rapids, MI

Spectrum Health Gerber Laboratory, Fremont, MI

Spectrum Health Kelsey Laboratory, Lakeview, MI

Spectrum Health Ludington Laboratory, Ludington, MI

Spectrum Health Pennock Laboratory, Hastings, MI

Spectrum Health Reed City Laboratory, Reed City, MI

Spectrum Health United Laboratory, Greenville, MI

Spectrum Health Zeeland Laboratory, Zeeland, MI


Flow Cytometry method is used to identify and isolate the immature platelets from the mature platelets based on size and the RNA and DNA content. The immature platelet fraction (IPF) is expressed as a proportional value of the total fluorescent platelet count.



CDM Code

Not available

Epic Test ID


Interface Order Alias

Not available

Mayo Access Code

Not available


Not available

Reviewed Date