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Test Code LAB1230491 ANA (Antinuclear Antibodies) Screen, Double Reflex

Test Name Alias

ANA | Antinuclear Antibody | ANA Screen | ENA | Extractable Nuclear Antigens | ANA Screen Double Reflex | 184

Clinical Information

Multiplex bead assay screens for anti-dsDNA, anti-Sm, anti-RNP, anti-SSA, anti-SSB, anti-Scl 70, anti-centromere, anti-Jo 1, anti-Sm/RNP, Ribosomal P, and chromatin. All positive ANA screens will reflex to Lab3540 ANA Hep 2 IFA and if positive at greater than or equal to 1:160 will automatically reflex to these specific ENA antibodies: anti-dsDNA, anti-Sm, anti-RNP, anti-SSA, anti-SSB, anti-Scl70, anti-centromere and anti-Jo 1. All antibodies included in screen are orderable as individual tests.
ANA Hep 2 IFA (LAB3540) will reflex to titer and pattern at 1:80

Icons & Photos

Not available

Collection Instructions

Specimen Collection: Blood

Container(s): Gold Top (Serum Separator-SST Gel)

Preferred Volume to Collect: 5.0 mL

Minimum Volume to Collect: 2.5 mL

Neonate Volume to Collect: 2.5 mL

Capillary collect ok: Yes

Microtainer acceptable: Yes

Collection Instructions:

  • After collection, gently invert tube 8-10 times.
  • Specimen should be processed within 2 hours – See Processing Instructions.

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Processed Specimen: Serum

Centrifuge/Spin: Yes

Aliquot: No

Processing Instructions:

  • Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.

Transport Temperature: Refrigerate

Rejection Criteria

Specimen not centrifuged within 2 hours

Quantity not sufficient

Reflex Information

Reflex approved by Medical Executive Committee. Please see link: Reflex Testing Document

Specimen Stability

Ambient: 12 hours

Refrigerate: 7 days

Frozen: aliquot serum for longer storage

Laboratory Retention: 7 days

Test Frequency

Available M-F, usual TAT 1-3 days

Reference Range


Performing Department


Performing Department Laboratory Location

Spectrum Health Regional Laboratory, Grand Rapids, MI


Multiplex Flow Assay, IFA


86038, 86039 if reflex IFA

CDM Code

Not available

Epic Test ID


Interface Order Alias


Mayo Access Code

Not available


Not available

Reviewed Date