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Test Code LAB1230571 Lyme Total Antibody

Important Note

Please refer to this blog post regarding Lyme Total Antibody. Due to reagent backorder, this test is temporarily being sent to Mayo Clinic Laboratories. Please order LAB1230745 until further notice.

Test Name Alias

Lyme Disease Serology | Lyme IgM/IgG | Borrelia burgdorferi | b. burgdorferi tick-borne

Clinical Information

For the qualitative detection of total (IgM/IgG) antibodies to Borrelia burgdorferi in human serum. All reactive and equivocal specimens will be tested with a second tier Wester Blot test. Positive second tier results are supportive evidence of infection with b. Burgdorferi. Diagnosis should be made based on the presence of B. Burgdorferi antibodies, history, symptoms,and other laboratory data. Non-Reactive first tier or negative second tier results should not be used to exclude borreliosis. 

Collection Instructions

Specimen Collection: Blood

Container(s): Gold Top (Serum Separator-SST Gel)

Preferred Volume to Collect: 5.0 mL

Minimum Volume to Collect: 1.0 mL

Neonate Volume to Collect: 1.0 mL

Capillary collect ok: Yes

Microtainer acceptable: Yes

Collection Instructions:

  • Gently invert gold tube(s) 8 - 10 times to mix clot activator with blood.
  • Specimen should be processed within 2 hours – See Processing Instructions.

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Processed Specimen: Serum

Centrifuge/Spin: Yes

Aliquot: No

Minimum Serum Volume: 1.0 mL

Processing Instructions:

  • Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.

Transport Temperature: Refrigerate

Rejection Criteria

Specimen not centrifuged within 2 hours

Specimen Stability

Ambient: 8 hours

Refrigerate: 7 days

Frozen: Aliquot serum for longer storage

Laboratory Retention: 7 days

Reflex Information

Reflex approved by Medical Executive Committee. Please see link: Reflex Testing Document

Positives will be sent to Mayo Medical Laboratories for Lyme Disease Antibody, Immunoblot

Test Frequency

Monday through Friday, turn-around time 1 to 3 days

Reference Range


Performing Department


Performing Department Laboratory Location

Spectrum Health Regional Laboratory (SHRL BW), Grand Rapids, MI


Multiplex Flow Assay



CDM Code


Epic Test ID


Interface Order Alias



Result name: 46248-1

Mayo Access Code


Reviewed Date