Sign in →

Epic Code LAB1230595 Hemoglobin Fractionation

Important Note

Tier 1 Testing:  Hemoglobin (HgB) Fractionation is performed at Spectrum Health Regional Lab and should be orderd first when assessing if hemoglobin variants and Thalassemias are present.

Beginning March 4, 2020: A CBC w/o differential will be ordered on all orders for Hemoglobin Fractionation that do not already have a CBC ordered in the past 30 days.

Test Name Alias

Hgb | Hgb Thalassemia | Hgb electrophoresis | Hemoglobinopathy | 8183

Interface Order Alias

1230595

Collection Instructions

Specimen Collection: Blood

Container(s): Full Lavender Top (EDTA) 3.0 mL 

Preferred Volume to Collect: 3.0 mL 

Minimum Volume to Collect: 1.0 mL

Neonate Volume to Collect: 1.0 mL

Capillary collect ok: Yes

Microtainer acceptable: Yes

Collection Instructions:

  • Gently invert lavender tube(s) 8 - 10 times after collection.
  • In cases where fractionation may be needed at a day or time when testing is not usually performed, the ordering provider must contact pathologist on call for approval.

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Processed Specimen: Whole Blood EDTA

 

Centrifuge/Spin: No

Aliquot: No

 

Transport Temperature: Ambient acceptable for first 24 hours.

Refrigerate if transport time will exceed 24 hours

Rejection Criteria

  • Specimen completely clotted
  • Greater than 7 days old

 

Specimen Stability

Ambient: 24 hours

Refrigerate: 1 week

Laboratory Retention: 7 days

Reflex Information

Link: Reflexes approved by MEC

  • CBC (LAB294) on all orders for Hemoglobin Frationation that do not already have a CBC ordered in the past 30 days
  • If Hgb S is presumptively identified, a Sickle Cell Screen will be performed as a reflex confirmatory test.
  • If Hgb S is suspected in an infant less than 6 months of age, Capillarys Hemoglobin Electrophoresis will be performed as a reflex confirmatory test.
  • If Hgb C is suspected, Capillarys Hemoglobin Electrophoresis will be performed as a reflex confirmatory test.
  • If Hgb A2 result is >10%, Capillarys Hemoglobin Electrophoresis will be performed as a reflex test to confirm Hgb E.

Test Frequency

Available Monday and Thursday, usual TAT 1- 4 days.

Reference Range

Hgb A2
1.5 – 3.5 %

 

Hgb F
≤ 0.9 %

Performing Department

IMMUNOCHEMISTRY

Performing Department Laboratory Location

Spectrum Health Regional Laboratory, Grand Rapids, MI

Methodology

Ion exchange high performance liquid chromatography (HPLC)

CPT

83021 HGB Fractionation

85027 CBC without Differential

CDM Code

80101551

80101894

Epic Test ID

1230100750;1230100453

Mayo Access Code

Unavailable for Mayo Access

Reviewed Date

12/13/2021

Beaker Names

Beaker Procedure Name: HEMOGLOBIN FRACTIONATION
Beaker Display Name: Hemoglobin (Hgb) Fractionation
BEAKER TEST NAME: CBC WITHOUT DIFFERENTIAL
BEAKER TEST REPORT NAME: CBC WITHOUT DIFFERENTIAL