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Epic Code LAB1230833 2,3-Dinor 11 Beta-Prostaglandin F2 Alpha, 24 Hour, Urine

Additional Codes

Mayo: 23BPT

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Screening for mast cell activation disorders including systemic mastocytosis using 24-hour urine specimens

Specimen Type

Urine


Ordering Guidance


A 24-hour urine collection is the preferred specimen type, but a random specimen is also acceptable for 2,3-dinor 11beta-prostaglandin F2 alpha; order 23BPR / 2,3-Dinor 11 Beta-Prostaglandin F2 Alpha, Random, Urine.

 

If the total volume provided is less than 500 mL, this test will be canceled and 23BPR ordered and performed.



Necessary Information


Specimen volume and duration are required.



Specimen Required


Patient Preparation: Patients taking aspirin or nonsteroidal anti-inflammatory drugs (NSAID) may have decreased concentrations of prostaglandin F2 alpha. If possible, discontinue for 2 weeks or 72 hours, respectively, prior to collecting a specimen.

Supplies: Aliquot Tube, 5 mL (T465)

Container/Tube: Plastic, 5-mL tube

Specimen Volume: 5 mL

Collection Instructions:

1. Collect urine for 24 hours.

2. No preservative preferred.

Additional Information: See Urine Preservatives-Collection and Transportation for 24-Hour Urine Specimens for multiple collections.


Specimen Minimum Volume

4 mL

Highlights

2,3-Dinor-11beta-prostaglandin F2 alpha (2,3 BPG) is elevated in the urine of patients with systemic mastocytosis (SM).

 

This test should be used as a screening test for SM.

 

When 2,3 BPG is used in combination with urinary leukotriene E4 and N-methyl histamine, the sensitivity for SM detection increases to 90%.

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Refrigerated (preferred) 14 days
  Frozen  30 days
  Ambient  8 hours

Reject Due To

  All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Cautions

Elevated levels of 2,3-dinor-11beta-prostaglandin F2 alpha (2,3 BPG) in urine are not specific for systemic mast cell disease and may be found in patients with angioedema, diffuse urticaria, or myeloproliferative diseases in the absence of diffuse mast cell proliferation.

 

Systemic mast cell disease is a heterogeneous disease, and some patients may not have elevated 2,3 BPG in urine.

Clinical Information

2,3-Dinor-11beta-prostaglandin F2 alpha (2,3 BPG) is the most abundant metabolic product of prostaglandins released by activated mast cells. Systemic mastocytosis (SM) is a disease in which clonally derived mast cells accumulate in peripheral tissues. Degranulation of these mast cells releases large amounts of histamines, prostaglandins, leukotrienes, and tryptase.

 

World Health Organization diagnostic criteria for SM require the presence of elevated mast cell counts on a bone marrow biopsy and 1 of the following minor criteria:

-Abnormal mast cell morphology

-KIT Asp816Val variant

-CD25-positive mast cells

-Serum tryptase greater than 20 ng/mL

 

Alternatively, SM diagnosis can be made with the presence of 3 minor criteria in the absence of abnormal bone marrow studies.

 

Measurement of mast cell mediators in blood or urine is less invasive and is advised for the initial evaluation of suspected cases. Elevated levels of serum tryptase, urinary N-methylhistamine, 2,3 BPG, or leukotriene E4 are consistent with the diagnosis of systemic mast cell disease.

Method Name

T23BP: Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

CRT24: Enzymatic Colorimetric Assay

Report Available

3 to 7 days

Reporting Name

2,3-dinor 11B-Prostaglandin F2a, U

Reference Values

<1802 pg/mg creatinine

Interpretation

Elevated urine 2,3-dinor-11beta-prostaglandin F2 alpha is consistent with systemic mastocytosis.

Method Description

2,3-Dinor-11beta-prostaglandin F2 alpha (2,3 BPG) is quantified in urine by liquid chromatography-tandem mass spectrometry.(Unpublished Mayo method)

 

Creatinine:

All 2,3 BPG concentrations are normalized to urine creatinine levels measured using a Roche cobas enzymatic method. The enzymatic method is based on the determination of sarcosine from creatinine with the aid of creatininase, creatinase, and sarcosine oxidase. The liberated hydrogen peroxide is measured via a modified Trinder reaction using a colorimetric indicator. Optimization of the buffer system and the colorimetric indicator enables the creatinine concentration to be quantified both precisely and specifically.(Package insert: Creatinine plus ver 2. Roche Diagnostics; V15.0 03/2019)

Day(s) Performed

Tuesday, Thursday

Clinical Reference

1. Gotlib J, Pardanani A, Akin C, et al: International Working Group-Myeloproliferative Neoplasms Research and Treatment (IWG-MRT) and European Competence Network on Mastocytosis (ECNM) consensus response criteria in advanced systemic mastocytosis. Blood. 2013 Mar 28;121(13):2393-2401. doi: 10.1182/blood-2012-09-458521

2. Butterfield JH: Increased leukotriene E4 excretion in systemic mastocytosis. Prostaglandins Other Lipid Mediat. 2010 Jun;92(1-4):73-76. doi: 10.1016/j.prostaglandins.2010.03.003

3. Roberts LJ II, Sweetman BJ, Lewis RA, Austen KF, Oates JA: Increased production of prostaglandin D2 in patients with systemic mastocytosis. N Engl J Med. 1980 Dec 11;303(24):1400-1404. doi: 10.1056/NEJM198012113032405

4. Metcalfe DD: Mastocytosis syndromes. In: Middleton E Jr, Reed CE, Ellis EF, et al. eds. Allergy Principles and Practice. Vol II. 4th ed. Mosby Yearbook Inc; 1993:1537-1551

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

84150

LOINC Code Information

Test ID Test Order Name Order LOINC Value
23BPT 2,3-dinor 11B-Prostaglandin F2a, U 94381-1

 

Result ID Test Result Name Result LOINC Value
CR_A Creatinine, 24 HR, U 2162-6
603460 2,3-dinor 11B-Prostaglandin F2a 94381-1
TM10 Collection Duration 13362-9
VL8 Urine Volume 3167-4
CR_24 Creatinine Concentration, 24 HR, U 20624-3

Profile Information

Test ID Reporting Name Available Separately Always Performed
T23BP 2,3-dinor 11B-Prostaglandin F2a No Yes
CRT24 Creatinine, 24 HR, U No Yes

Urine Preservative Collection Options

Note: The addition of preservative or application of temperature controls must occur within 4 hours of completion of the collection.

 

Ambient

No

Refrigerate

Preferred

Frozen

OK

50% Acetic Acid

OK

Boric Acid

OK

Diazolidinyl Urea

No

6M Hydrochloric Acid

No

6M Nitric Acid

No

Sodium Carbonate

OK

Thymol

No

Toluene

No