Sign in →

Epic Code LAB1230860 FLT3 Mutation Analysis

Test Name Alias

FLT3

Interface Order Alias

1230860

Clinical Information

Qualitative PCR-based assay was performed on genomic DNA to detect two major classes of activating FLT3 mutations: internal tandem duplications (ITD) and tyrosine kinase domain (TKD) point mutation (D835 and/or I836). FLT3-ITD occurs in approximately 30% of acute myeloid leukemia (AML) patients and is associated with adverse prognosis. This prognostic influence may be further modified by FLT3-ITD allelic ratio. Targeted therapies were approved by the FDA for adult patients harboring FLT3-ITD and/or TKD mutation. The limit of detection of this assay is about 5% allele frequency. False positive or false negative results may occur for reasons that include genetic variants, large ITD size, and somatic heterogeneity of the sample. This test should not be used to monitor residual disease following treatment. 

Collection Instructions

Submit only ONE of the following specimens:

 

Specimen Collection: Bone Marrow (Preferred)

 

Container(s): Lavender top (EDTA) tube

Preferred Volume to Collect: 3 mL

Minimum Volume to Collect: 1 mL

Neonate Volume to Collect: 1 mL

 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Transport Temperature: Ambient

Specimen Collection: Blood

 

Container(s): Lavender top (EDTA) tube

Preferred Volume to Collect: 3 mL

Minimum Volume to Collect: 1 mL

Neonate Volume to Collect: 1 mL

Capillary collect ok? Yes

Microtainer acceptable: Yes

 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

  • Processed Specimen: Whole Blood in Lavender tube
  • Centrifuge/Spin: No
  • Aliquot: Yes

Transport Temperature: Ambient

 

Specimen Collection: Paraffin block

If applicable, the ATL Surgical Specimen form completed by the Pathologist should be submitted with the paraffin embedded tissue.

 

 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Transport Temperature: Ambient

Specimen Stability

Ambient: 72 Hours (24 hours preferred)

Refrigerated: 7 days

Test Frequency

Run as needed.

STAT and Routine: 48 hours from receipt into Molecular Diagnostics Laboratory

Reference Range

Normal: Not Detected

Abnormal: Dectected

Performing Department

Molecular Diagnostics

Performing Department Laboratory Location

Corewell Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Qualitative PCR-based assay

CPT

81245, 81246

CDM Code

Unavailable

Epic Test ID

1230102538, 1230102538

Reviewed Date

6/22/2023