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Epic Code LAB175 HIV Quick Test

Important Note

Alere Determine HIV-1/2 Ag/AB Combo (HIV Quick) is not intended for newborn screening or for use with cord blood specimens or specimens from individuals less than 12 years of age.

Spectrum Health Reference Laboratory: HIV Quick will be canceled and reordered as HIV 1/2 Antibody EIA (see reflex information below)

 

Test cannot be ordered as an add-on

Test Name Alias

Rapid HIV Screen | 245

Interface Order Alias

10729

Clinical Information

Includes tests for two HIV infection markers, HIV-1 p24 antigen and HIV 1/2 antibody. HIV-1 p24 antigen is an earlier marker of HIV infection which may become positive before detectable HIV antibody appears.

 

Specimens with positive results for HIV-1 p24 antigen and/or HIV 1/2 antibody will be further evaluated with HIV 1/HIV 2 Ab Ag Diagnostic (Roche).

Collection Instructions

Specimen Collection: Blood

 

Container(s): 5.0 mL Gold Top (Serum Separator-SST Gel)

Preferred Volume to Collect: 5.0 mL

Minimum Volume to Collect: 1.0 mL

Neonate Volume to Collect: 1.0 mL

Capillary collect ok? Yes

Microtainer acceptable: Yes (2 microtainers)

 

Collection Instructions:

  • Specimens from other sources are not acceptable.
  • Gold tube: Gently invert 5 times to mix clot activator with blood.
  • Specimen should be processed within 2 hours – See Processing Instructions. 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Processed Specimen: Serum

Centrifuge/Spin: Yes

Aliquot: Yes

 

Processing Instructions:

  • Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.
  • Frozen specimen can the thawed no more than 3 times.
  • All frozen specimens must be centrifuged at 10,000g for 5 min at room temperature.
  • Carefully remove the 50 μL test sample from the supernatant.  If a lipid layer is formed on the surface of the liquid, ensure that the sample is taken from the clear liquid below that layer.

Transport Temperature: Refrigerate

Specimen Stability

Ambient: 48 hours

Refrigerate: 7 days

Frozen: aliquot serum for longer storage

Laboratory Retention: 72 hours

Reflex Information

Link: Reflex approved by MEC

Test Frequency

Available 24 hours, usual TAT 1 hour.

Reference Range

Negative

 

Laboratory Critical Values Table

 

The performance of this assay has not been established for those under 12 years old.

Performing Department

CHEMISTRY

Performing Department Laboratory Location

Spectrum Health Regional Laboratory, Grand Rapids, MI
Spectrum Health Big Rapids Laboratory, Big Rapids, MI
Spectrum Health Blodgett Laboratory, Grand Rapids, MI
Spectrum Health Gerber Laboratory, Fremont, MI
Spectrum Health Kelsey Laboratory, Lakeview, MI
Spectrum Health Ludington Laboratory, Ludington, MI
Spectrum Health Pennock Laboratory, Hastings, MI
Spectrum Health Reed City Laboratory, Reed City, MI
Spectrum Health United Laboratory, Greenville, MI

Methodology

Immunochromatographic

CPT

86703-LAB-HIV QUICK TEST
87899-LAB HIV-1 P24 AG

CDM Code

4054082

4054084

Epic Test ID

1230100790

LOINC

HIV Quick HIV-1 p24 Ag: 9821-0

HIV Quick HIV 1/2 Ab: 7918-6

HIV Quick Disclaimer: 8251-1

Beaker LOINC

HIV QUICK DISCLAIMER: 8251-1
HIV QUICK HIV 1/2 AB: 7918-6
HIV QUICK HIV-1 P24 AG: 9821-0

Reviewed Date

3/23/2022

Beaker Names

Beaker Procedure Name: RAPID HIV SCREEN
Beaker Display Name: HIV Quick Test
BEAKER TEST NAME: HIV QUICK TEST
BEAKER TEST REPORT NAME: HIV Quick Test

Beaker Synonyms

No synonym on file

Beaker Location, Container and Temperature

BW CHEMISTRY: GOLD TOP SST TUBE R (Preferred)-Refrigerated
BL CHEMISTRY: GOLD TOP SST TUBE R (Preferred)-Refrigerated
BR CHEMISTRY: GOLD TOP SST TUBE R (Preferred)-Refrigerated
GM CHEMISTRY: GOLD TOP SST TUBE R (Preferred)-Refrigerated
KL CHEMISTRY: GOLD TOP SST TUBE R (Preferred)-Refrigerated
LH CHEMISTRY: GOLD TOP SST TUBE R (Preferred)-Refrigerated
PH CHEMISTRY: GOLD TOP SST TUBE R (Preferred)-Refrigerated
RC CHEMISTRY: GOLD TOP SST TUBE R (Preferred)-Refrigerated
UN CHEMISTRY: GOLD TOP SST TUBE R (Preferred)-Refrigerated
ZH CHEMISTRY: GOLD TOP SST TUBE R (Preferred)-Refrigerated