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Test Code LAB2111011 BCR-ABL1 t[9;22] RT- PCR

Test Name Alias

BCR-ABL1 t[9 |22] RT-PCR | 9011

Clinical Information

The BCR-ABL1 assay uses real-time rtPCR to detect the BCR-ABL1 major (p210) and minor (p190) transcripts from the t(9;22) translocation, which can be used to monitor minimal residual disease in patients with CML.

Specimen Requirements

Submit only one of the following specimens:

 

Specimen Type: Whole Blood EDTA
Container/Tube: Lavender Top (EDTA)
Minimum volume: 6 mL
Collection Instructions: For optimal results, specimen should be received and processed within 24 hours of collection.

 

Specimen Type: Bone Marrow
Container/Tube: Lavender Top (EDTA)
Minimum volume: 6 mL
Collection Instructions: For optimal results, specimen should be received and processed within 24 hours of collection.

Specimen Transport Temperature

If received within 24 hours of collection, specimen can be transported at ambient temperature or refrigerated.

If transport will exceed 24 hours, recommend specimen be refrigerated.

Specimen Stability

Ambient: <24 hours

Refrigerate: <72 hours

Laboratory Retention: Extracted RNA retained greater than 1 year

Test Frequency

Available once per week, usual TAT less than 7 days

Reference Range

An interpretative report will be provided.

Performing Department

Molecular Diagnostics

Performing Department Laboratory Location

Spectrum Health Regional Laboratory (SHRL) – Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Real-Time Quantitative Polymerase Chain Rection (RT-qPCR)

CPT

81206, 81207

CDM Code

3900299, 3900300

Interface Order Alias

11111

Mayo Access Code

SHO9011

LOINC

Specimen Source: 31208-2

Specimen ID: 8251-1

BCR-ABL t[9:22]: 63422-0

BCR-ABL t[9:22] RT-PCR Ratio: 69380-4

BCR-ABL Log Reduction: 50195-7

BCR-ABL comm: 8251-1

Reviewed Date

7/11/2016