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Epic Code LAB2111349 ALK (2p23) Rearrangement by FISH

Test Name Alias

ALK (2p23) Rearrangement by FISH | FISH for ALK Break Apart | 943

Interface Order Alias

11363

Clinical Information

Fluorescence in situ hybridization (FISH) using DNA probes to detect ALK (Anaplastic Lymphoma Kinase) rearrangements at 2p23 that occur in t(2;5) and its variants.

 

Mandatory Reflex Information:

  • All lung adenocarcinomas, EGFR/ALK will be performed

Icons & Photos

Green Sodium Heparin

Bone Marrow & Peripheral Blood

 

Specimen Requirements

Multiple specimen collection types. Submit only one:

Bone Marrow

Specimen Collection: Bone Marrow

Container(s): Green Top (Sodium Heparin)

Preferred volume to collect: 5.0 mL

Minimum volume to collect: 1.0 mL

Neonate volume to collect: 1.0 mL

Collection Instructions:

  • Collect bone marrow and transfer marrow from syringe to plain green top sodium heparin tube. 

 

Peripheral Blood

Specimen Collection: Peripheral blood
Container/Tube: Green Top (Sodium Heparin)

Preferred volume to collect: 8.0 mL

Minimum volume to collect: 0.5 mL

Neonate volume to collect: 0.5 mL

Collection Instructions:

  • Peripheral blood may be substituted for Bone Marrow if circulating blasts are present 
  • Collect peripheral blood into a plain green top sodium heparin tube. 
  • Keep sample at room temperature and send to the Lab immediately. 

 

FFPE Tissue

Specimen Collection: Formalin-fixed, paraffin-embedded (FFPE) tissue

Container(s): Block

Preferred Volume to Collect: 4 unstained slides cut between 4 and 6 microns thick

Collection Instructions:

  • Include an H&E slide scribed by Pathologist and Surgical Specimen Testing order sheet.
  • Place specimen in 10% Formalin and order Tissue Pathology in addition to ALK (2p23) Rearrangement by FISH order.

 

FNA

Specimen Collection: Fine Needle Aspirate (FNA, such as lymph node)

Collection Instructions: Order FNA Cytology (refer to test code 202 for collection instructions) in addition to ALK (2p23) Rearrangement by FISH order.

 

Pleural Fluid

Specimen Collection:  Pleural Fluid

Collection instructions:  Place specimen in sterile container and order Medical Cytology in addition to ALK (2p23) Rearrangement by FISH order.

 

Fresh Tumor

Specimen Collection: Fresh Tumor: 1.0 cm³ biopsy specimen under sterile conditions.

Container(s): Sterile container

Collection Instructions: 

  • Place in sterile container with either tissue culture media or transport media and an antibiotic such as Pen/Strep or Gentamicin.
  • Tissue may be stored overnight in medium at 4° C.

 

 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Transport Temperature: Ambient (Fresh Tumor, should be refrigerated temperature)

If ambient temperature exceeds 80°F or is below 40°F, please send in an insulated container. All samples should be directed to the laboratory as soon as possible.

Specimen Stability

Ambient: 3 days for fresh specimens (beyond 3 days, specimen viability will be detemined by Cytogenetics Lab)

Laboratory Retention: primary specimens 4 weeks; frozen pellets retained greater than 1 year

Test Frequency

Test Set Up: Monday – Friday 8:00 am – 5:00 pm, Saturday 8:00 am – 3:00 pm.

Technologist on call for Sunday set up.

Final report: 2 – 5 days.

Reference Range

An interpretative report will be provided.

Performing Department

CYTOGENETICS

Performing Department Laboratory Location

Spectrum Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Performed by fluorescence in situ hybridization (FISH). This test was developed and its performance characteristics determined by Spectrum Health Cytogenetics. It has not been cleared or approved by the U.S. FDA. The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes.

CPT

88368-MORPH ISH INTL SINGLE PRB

88369-MORPH ISH ADDTL SINGLE PRB

CDM Code

80202698, 80202699

Epic Test ID

1230100310

LOINC

Specimen Type: 66746-9

Indication Provided: 42349-1

Result: in process

Interpretation: 59050-5

Comments: 69965-2

Beaker LOINC

LOINC Not available

Reviewed Date

10/22/2021

Beaker Names

Beaker Procedure Name: ALK (2P23) REARRANGEMENT BY FISH
Beaker Display Name: ALK (2p23) Rearrangement by FISH (Lab Use Only)
BEAKER TEST NAME: ALK (2P23) REARRANGEMENT BY FISH
BEAKER TEST REPORT NAME: ALK (2p23) Rearrangement by FISH

Beaker Synonyms

ALK REARRANGEMENT

Beaker Collection and Specimen Handling

BEAKER COLLECTION: AP Ambient

Beaker Location, Container and Temperature

LC CYTOGENETICS: 6 ML NA HEPARIN TUBE A (Preferred)-Ambient
LC CYTOGENETICS: CYTOGENETICS MEDIA (Acceptable)-Ambient
LC CYTOGENETICS: MISCELLANEOUS (Acceptable)-Ambient
LC CYTOGENETICS: STERILE CONTAINER A (Acceptable)-Ambient