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Epic Code LAB2111355 BCR and ABL1 t(9;22) by FISH

Test Name Alias

BCR and ABL1 t(9;22) by FISH | BCR and ABL1 Dual Fusion | 946

Interface Order Alias

11369

Clinical Information

Fluorescence in situ hybridization (FISH) using BCR/ABL 1 probe is useful in the identification of the Philadelphia chromosome in patients with CML, ALL, or AML. FISH is also useful in monitoring remission in patients.

 

Routine chromosome analysis is performed on all positive diagnostic samples and is recommended on all bone marrow specimens to exclude additional abnormalities not identified by the specified FISH probe(s).

Collection Instructions

Multiple specimen collection types. Submit only one:

 

Specimen Collection: Bone Marrow

Container(s): Green Top (Sodium Heparin)

Preferred volume to collect: 5.0 mL

Minimum volume to collect: 1.0 mL

Neonate volume to collect: 1.0 mL

Collection Instructions:

  • Collect bone marrow and transfer marrow from syringe to plain green top sodium heparin tube. 
  • A bone marrow sample is the preferred sample type for testing.

 

Specimen Collection: Peripheral blood
Container/Tube: Green Top (Sodium Heparin)

Preferred volume to collect: 8.0 mL

Minimum volume to collect: 0.5 mL

Neonate volume to collect: 0.5 mL

Collection Instructions:

  • Peripheral blood may be substituted for Bone Marrow if circulating blasts are present 
  • Collect leukemic peripheral blood into a plain green top sodium heparin tube. 
  • Keep sample at room temperature and send to the Lab immediately. 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Spin: No

Aliquot: No

 

Transport Temperature: Ambient

 

If ambient temperature exceeds 80°F or is below 40°F, please send in an insulated container. All samples should be directed to the laboratory as soon as possible.

Specimen Stability

Ambient: 3 days for fresh specimens (beyond 3 days, specimen viability will be detemined by Cytogenetics Lab)

Laboratory Retention: primary specimens 4 weeks; frozen pellets retained greater than 1 year

Test Frequency

Test Set Up: Monday – Friday 8:00 am – 5:00 pm, Saturday 8:00 am – 3:00 pm.

Technologist on call for Sunday set up.

Final report: 2-3 days.

Reference Range

An interpretative report will be provided.

Performing Department

CYTOGENETICS

Performing Department Laboratory Location

Spectrum Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Performed by fluorescence in situ hybridization (FISH). This test was developed and its performance characteristics determined by Spectrum Health Cytogenetics. It has not been cleared or approved by the U.S. FDA. The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes.

CPT

88275, 88271 x2

CDM Code

4148275, 4148271

Epic Test ID

1230100374

LOINC

Specimen Type: 66746-9

Indication Provided: 42349-1

Result: in process

Interpretation: 59050-5

Comments: 69965-2

Beaker LOINC

LOINC Not available

Reviewed Date

10/22/2021

Beaker Names

Beaker Procedure Name: BCR AND ABL1 T(9;22) BY FISH
Beaker Display Name: BCR and ABL1 t(9;22) by FISH
BEAKER TEST NAME: BCR AND ABL1 T(9;22) BY FISH
BEAKER TEST REPORT NAME: BCR and ABL1 t(9;22) by FISH

Beaker Synonyms

BCR/ABL1

Beaker Collection and Specimen Handling

BEAKER COLLECTION: AP Ambient

Beaker Location, Container and Temperature

LC CYTOGENETICS: 6 ML NA HEPARIN TUBE A (Preferred)-Ambient
LC CYTOGENETICS: CYTOGENETICS MEDIA (Acceptable)-Ambient
LC CYTOGENETICS: MISCELLANEOUS (Acceptable)-Ambient
LC CYTOGENETICS: STERILE CONTAINER A (Acceptable)-Ambient