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Epic Code LAB2111400 Leukemia/Lymphoma/Myeloma Panel by Flow Cytometry

Test Name Alias

Flow Cytometry | Leukemia or Non-Hodgkins Lymphoma Panel by Flow Cytometry | Myeloma | LeukNHLPanel | NHL | Leukemia/Lymphoma Phenotype | ATL | B Cell All Minimal Residual Disease (MRD) | Leukemia Immunophenotyping | Neuroblastoma | 910 | CAR-T | CART | Hairy Cell Leukemia | Mantle Cell Lymphoma | Chronic Lymphocytic Leukemia | Monoclonal Plasma Cells | MDS | AML | Acute Myeloid Leukemia | Lymphoproliferative Disorder | Acute Lymphoblastic Leukemia | ALL

Interface Order Alias

11414

Clinical Information

This flow cytometry assay involves the staining of white blood cells using monoclonal antibodies in order to classify expression of antigens on the surface, in the cytoplasm, or within the nucleus of the cell.  The resulting CD expression or immunophenotype is used by the hematopathologist to aid in the identification and diagnosis of various malignancies as well as to follow up after treatment. Standard screening panels of antibodies are sufficiently comprehensive to address the clinical considerations for the patient samples submitted (i.e., the presence or absence of detectable leukemia, lymphoma, plasma cell myeloma, or neuroblastoma).

Icons & Photos

Green Top

Sodium Heparin

(Bone Marrow, Whole Blood, or some fluids)

Lavender top 

EDTA

(Bone Marrow, or Whole Blood)

Hanks Salt Solution

(Bone cores, tissues, FNA or biopsies)

Sterile continer or tube

(Some fluids and CSF)

 

Collection Instructions

Flow Cytometry: Specimens placed in formalin, fixed slides, and tissue touch prep slides are NOT acceptable.

Submit only one of the following specimens:


Specimen Type: Bone Marrow
Container/Tube: Green Top (Sodium Heparin) or Lavender top (EDTA)
Volume: 2.0 - 3.0 mL
Minimum volume: 0.5 mL
Collection Instructions: Store ambient


Specimen Type: Whole Blood
Container/Tube: Green Top (Sodium Heparin) or Lavender top (EDTA)
Volume: 5.0 mL
Minimum volume: 0.5 mL

Pediatric volume: 1.0 mL
Collection Instructions: Store ambient


Specimen Type: Bone cores, tissues, and fine needle aspirates (FNA) or biopsies
Container/Tube: Place specimen in Hanks' Balanced Salt Solution. Using sterile saline is acceptable.
Collection Instructions: Store refrigerate


Specimen Type: Fluids
Container/Tube: Sterile container or Green Top (Sodium Heparin)
Collection Instructions: Store refrigerate


Specimen Type: Cerebrospinal Fluid (CSF)
Container/Tube: Sterile container
Volume: 2.0 mL
Minimum volume: 0.5 mL
Collection Instructions: Store refrigerate. Hand deliver - do not send through tube system.

 

Lab Staff Instructions

  • Yellow Top (ACD Solution) is an acceptable, but not preferred, collection container for bone marrow and whole blood. Do not discard.

Specimen Stability

Ambient (bone marrow and blood): 48 hours*

Refrigerate: 48 hours*

Laboratory Retention: 7 days

*Flow cytometry: Specimens >48 hours will still be run and reported

Reflex Information

Leukemia/Lymphoma Immunophenotyping Panel Reflex:

This is a standard screen for Leukemia, Lymphoma and Multiple Myeloma. Appropriate add on or reflex testing will be added at the discretion of the pathologist.

Test Frequency

Flow Cytometry:

Monday – Friday 7:00am – 3:30pm, usual TAT 3 days.

Saturday – Sunday 8:00am – 12:30pm

Reference Range

Flow cytometry: See report: See patient chart report for interpretation

Performing Department

FLOW CYTOMETRY

Performing Department Laboratory Location

Spectrum Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Flow Cytometry

CPT

Flow Cytometry

88184-LAB FLOW CYTO 1ST MARKER,               
88185-LAB FLOW CYTO EA ADD MKR (x each)

 

First cell surface marker will be billed with CPT code 88184. Each additional surface marker will be billed with CPT code 88185. The quantity of surface markers varies with each patient case and will be billed accordingly based on quantity performed. Corresponding professional fees will also apply.

CDM Code

4028304, 4028305

Epic Test ID

1230100882

Beaker LOINC

LOINC Not available

Reviewed Date

7/31/2021

Beaker Names

Beaker Procedure Name: LEUKEMIA/LYMPHOMA/MYELOMA PANEL BY FLOW CYTOMETRY
Beaker Display Name: Leukemia/Lymphoma/Myeloma Panel by Flow Cytometry
BEAKER TEST NAME: LEUKEMIA/LYMPHOMA/MYELOMA PANEL BY FLOW CYTOMETRY
BEAKER TEST REPORT NAME: Leukemia/Lymphoma/Myeloma Panel by Flow Cytometry

Beaker Synonyms

LEUKEMIA/NHL/MYELOMA|NHL|MYLOMA

Beaker Collection and Specimen Handling

BEAKER COLLECTION: AP LOOK+LOOK

Beaker Location, Container and Temperature

LC FLOW CYTOMETRY: 2 ML GREEN NA HEPARIN TUBE A (Preferred)-Ambient
LC FLOW CYTOMETRY: 3 ML LAVENDER TOP TUBE A (Acceptable)-Ambient
LC FLOW CYTOMETRY: HANK’S SOLUTION (Acceptable)-Refrigerated
LC FLOW CYTOMETRY: STERILE CONTAINER A (Acceptable)-Refrigerated