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Epic Code LAB2111412 N-MYC (2p24) Amplification by FISH

Test Name Alias

N-MYC (2p24) Amplification by FISH | 968

Interface Order Alias

11426

Clinical Information

Fluorescence in situ hybridization (FISH) using N-MYC (NMI) (2p24.1) DNA probe to identify amplification of the the N-myc genomic sequences found in some neuroblastomas and small cell lung carcinomas.

 

Routine chromosome analysis is performed on all diagnostic samples and is recommended on all bone marrow specimens to exclude any abnormalities not identified by the specified FISH probe(s).

Collection Instructions

Multiple specimen collection types. Submit only one.

 

Specimen Collection: Bone Marrow

Container(s): Green Top (Sodium Heparin)

Preferred volume to collect: 5.0 mL

Minimum volume to collect: 1.0 mL

Neonate volume to collect: 1.0 mL

Collection Instructions:

  • Collect bone marrow and transfer marrow from syringe to plain green top sodium heparin tube.

 

Specimen Collection: Formalin-fixed, paraffin-embedded (FFPE) tissue

Container(s): Block

Preferred Volume to Collect: 4 unstained slides cut between 4 and 6 microns thick

Collection Instructions:

  • Include an H&E slide scribed by Pathologist and Surgical Specimen Testing order sheet.
  • Place specimen in 10% Formalin and order Tissue Pathology in addition to N-MYC (2p24) Amplification by FISH order.

 

Specimen Collection: Fine Needle Aspirate (FNA, such as lymph node)

Collection Instructions: Order FNA Cytology (refer to test code 202 for collection instructions) in addition to N-MYC (2p24) Amplification by FISH order.

 

 

Specimen Collection:  Pleural Fluid

Collection instructions:  Place specimen in sterile container and order Medical Cytology in addition to N-MYC (2p24) Amplification by FISH order.

 

 

Specimen Collection: Fresh Tumor: 1.0 cm³ biopsy specimen under sterile conditions.

Container(s): Sterile container

Collection Instructions: 

  • Place in sterile container with either tissue culture media or transport media and an antibiotic such as Pen/Strep or Gentamicin.
  • Tissue may be stored overnight in medium at 4° C.

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Spin: No

Aliquot: No

 

Transport Temperature: Ambient (Fresh Tumor, should be refrigerated temperature)

If ambient temperature exceeds 80°F or is below 40°F, please send in an insulated container. All samples should be directed to the laboratory as soon as possible.

Specimen Stability

Ambient: 3 days for fresh specimens (beyond 3 days, specimen viability will be detemined by Cytogenetics Lab)

Laboratory Retention: primary specimens 4 weeks; frozen pellets retained greater than 1 year

Test Frequency

Test Set Up: Monday – Friday 8:00 am – 5:00 pm, Saturday 8:00 am – 3:00 pm.

Technologist on call for Sunday set up.

Final report: 2-3 days. 2 – 5 days for paraffin embedded specimens

Reference Range

An interpretive report will be provided.

Performing Department

CYTOGENETICS

Performing Department Laboratory Location

Spectrum Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Performed by fluorescence in situ hybridization (FISH).

 

This test was developed and its performance characteristics determined by Spectrum Health Cytogenetics Laboratory. It has not been cleared or approved by the US FDA. The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes.

CPT

88275, 88271 x2

CDM Code

4148275, 4148271

Epic Test ID

1230100978

LOINC

Specimen Type: 66746-9

Indication Provided: 42349-1

Result: in process

Interpretation: 59050-5

Comments: 69965-2

Beaker LOINC

LOINC Not available

Reviewed Date

10/22/2021

Beaker Names

Beaker Procedure Name: N-MYC (2P24) AMPLIFICATION BY FISH
Beaker Display Name: N-MYC (2P24) Amplification by FISH (Lab Use Only)
BEAKER TEST NAME: N-MYC (2P24) AMPLIFICATION BY FISH
BEAKER TEST REPORT NAME: N-MYC (2p24) Amplification by FISH

Beaker Synonyms

N-MYC AMPLICATION

Beaker Collection and Specimen Handling

BEAKER COLLECTION: AP Ambient

Beaker Location, Container and Temperature

LC CYTOGENETICS: 6 ML NA HEPARIN TUBE A (Preferred)-Ambient
LC CYTOGENETICS: CYTOGENETICS MEDIA (Acceptable)-Ambient
LC CYTOGENETICS: MISCELLANEOUS (Acceptable)-Ambient
LC CYTOGENETICS: STERILE CONTAINER A (Acceptable)-Ambient