Epic Code LAB2111414 Oxidative Burst by Flow Cytometry
Test Name Alias
DHR | CGD | Chronic Granulomatous Disease | Nitro Blue | Dihydrorhodamine 123 | Neutrophil Oxidative Burst
Interface Order Alias
11428
Clinical Information
Oxidative Burst by Flow Cytometry uses DHR (Dihydrorhodamine 123) labeled neutrophils stimulated by PMA to detect CGD patients, carriers, and may suggest the genotype of the CGD patients by using the NOI (Neutrophil Oxidative Index).
Collection Instructions
Collect only Monday through Friday
Specimen Collected: Blood
Container(s): Lavender top (EDTA) or Green top (Sodium Heparin)
Preferred Volume to Collect: 3.0 mL
Minimum Volume to Collect: 1.0 mL
Neonate Volume to Collect: 0.5 mL
Capillary collect ok? No
Microtainer acceptable: Yes
Collection Instructions:
- Lavender top is preferred.
- After collection, gently invert tube 8-10 times.
Processing Instructions (Laboratory, Outpatient or Off-site collection)
- Processed Specimen: Whole Blood
- Centrifuge/Spin: No
- Aliquot: No
- Transport Temperature: Ambient
Specimen Stability
Ambient: 48 hours from collection
Refrigerated: Unacceptable
Frozen: Unacceptable
Laboratory Retention: Specimen must remain ambient at all times and testing must be performed within 48 hours of collection, within 24 hours is optimal.
Test Frequency
Monday – Friday, usual TAT 2 days.
Test not available on weekends or holidays.
Reference Range
An interpretative report will be provided. Normal = NOI (Neutrophil Oxidative Index) ≥30
Performing Department
FLOW CYTOMETRY
Performing Department Laboratory Location
Spectrum Health Advanced Technology Laboratory (ATL), Grand Rapids, MI
Methodology
Flow Cytometry
CPT
86352
CDM Code
80102244
Epic Test ID
1230101009
Beaker LOINC
LOINC Not available
Reviewed Date
7/31/2021
Beaker Names
Beaker Procedure Name: OXIDATIVE BURST BY FLOW
CYTOMETRY
Beaker Display Name: Oxidative Burst by Flow
Cytometry
BEAKER TEST NAME: OXIDATIVE BURST BY FLOW
CYTOMETRY
BEAKER TEST REPORT NAME: Oxidative Burst by Flow
Cytometry
Beaker Synonyms
No synonym on file
Beaker Collection and Specimen Handling
BEAKER COLLECTION: AP Ambient LOOK+LOOK
Beaker Location, Container and Temperature
LC FLOW CYTOMETRY: 4 ML GREEN NA HEPARIN TUBE (Preferred)-Ambient