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Epic Code LAB2111414 Oxidative Burst by Flow Cytometry

Important Note

Only Collect this test Monday through Friday and is not available on weekends and holidays.

Test Name Alias

DHR | CGD | Chronic Granulomatous Disease | Nitro Blue | Dihydrorhodamine 123 | Neutrophil Oxidative Burst

Interface Order Alias

11428

Quick Collect

AP Ambient LOOK+LOOK

Clinical Information

Oxidative Burst by Flow Cytometry uses DHR (Dihydrorhodamine 123) labeled neutrophils stimulated by PMA to detect CGD patients, carriers, and may suggest the genotype of the CGD patients by using the NOI (Neutrophil Oxidative Index).

Collection Instructions

Collect only Monday through Friday

 

Specimen Collected: Blood

Container(s): Lavender top (EDTA) or Green top (Sodium Heparin)

Preferred Volume to Collect: 3.0 mL

Minimum Volume to Collect: 1.0 mL

Neonate Volume to Collect: 0.5 mL

Capillary collect ok? No

Microtainer acceptable: Yes

 

Collection Instructions:

  • Lavender top is preferred.
  • After collection, gently invert tube 8-10 times.

 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

  • Processed Specimen: Whole Blood
  • Centrifuge/Spin: No
  • Aliquot: No
  • Transport Temperature: Ambient

Specimen Stability

Ambient: 48 hours from collection

Refrigerated: Unacceptable

Frozen: Unacceptable

Laboratory Retention: Specimen must remain ambient at all times and testing must be performed within 48 hours of collection, within 24 hours is optimal.

Test Frequency

Monday – Friday, usual TAT 2 days.

Test not available on weekends or holidays.

Reference Range

An interpretative report will be provided. Normal = NOI (Neutrophil Oxidative Index) ≥30 

Performing Department

FLOW CYTOMETRY

Performing Department Laboratory Location

Corewell Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Flow Cytometry

Epic Test ID

1230101009

Reviewed Date

7/31/2021