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Test Code LAB2111414 Oxidative Burst by Flow Cytometry

Important Note

This test requires two specimens: one from the patient and one from an adult control.

Test Name Alias

Oxidative Burst by Flow Cytometry |Nitro Blue by Flow Cytometry | CGD | Chronic Granulomatous Disease | 917

Collection Instructions

Submit the following TWO specimens:

1. From the patient

2. From a healthy, unrelated adult

 

1. From the Patient

Specimen Collected: Blood

 

Container(s): Green top (Sodium Heparin)

Preferred Volume to Collect: 4.0 mL

Minimum Volume to Collect: 2.0 mL

Neonate Volume to Collect: 1.0 mL

Capillary collect ok?

Microtainer acceptable:

 

Collection Instructions:

  • After collection, gently invert tube 8-10 times.
  • Label specimen with barcode label or with 2 patient identifiers

 

2. From Adult Control

Specimen Collected: Blood

 

Container(s): Green top (Sodium Heparin)

Preferred Volume to Collect: 4.0 mL

Minimum Volume to Collect: 2.0 mL

 

Collection Instructions:

  • Draw from a healthy, unrelated adult, drawn at the same time as the patient sample
  • After collection, gently invert tube 8-10 times.
  • Clearly label the outermost label of the specimen “Normal Control”

 

Note: Specimens must be received before 10 am to be performed that day. Testing must be performed within 24 hours of collection. Test not available on weekends or holidays.

Processing Instructions (Laboratory, Outpatient or Off-site collection)

For BOTH Specimens

Processed Specimen: Whole Blood

 

Centrifuge/Spin: No

Aliquot: No

 

Transport Temperature: Ambient

 

Note: Specimens must be received before 10 am to be performed that day. Testing must be performed within 24 hours of collection. Test not available on weekends or holidays.

Specimen Stability

Ambient: 24 hours from collection

Refrigerated: Unacceptable

Frozen: Unacceptable

Laboratory Retention: Specimen must remain ambient at all times and testing must be performed within 24 hours of collection.

Test Frequency

Monday – Friday, usual TAT 3 days.

Specimens must be received before 10 am to be performed that day. Testing must be performed within 24 hours of collection. Test not available on weekends or holidays.

Reference Range

An interpretative report will be provided.

Performing Department

Flow Cytometry

Performing Department Laboratory Location

Spectrum Health Regional Laboratory (SHRL) – Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Flow Cytometry

CPT

86352

CDM Code

4084273

Interface Order Alias

11428

Reviewed Date

6/19/2018