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Epic Code LAB2111431 T-Cell Acute Lymphocytic Leukemia (T-ALL) Probe Set by FISH

Test Name Alias

T Cell Acute Lymphocytic Leukemia (T-ALL) Probe Set by FISH | 996

Interface Order Alias

11445

Quick Collect

AP Ambient

Clinical Information

This probe panel detects specific numerical and structural chromosome abnormalities commonly associated with T-lineage acute lymphoblastic leukemia.

Routine chromosome analysis is performed on all diagnostic samples and is recommended on all bone marrow specimens to exclude abnormalities not identified by the specified FISH probe(s).

Panel Information

Panel testing includes:

  • 14q11.2 rearrangement, TCRAD break apart
  • 7q34 rearrangement, TCRB break apart
  • 9p21, CDKN2A
  • 11q23 rearrangement, MLL (KMT2A)
  • 5q35 rearrangement, TLX3 break apart
  • t(9;22), BCR/ABL

Collection Instructions

Multiple specimen collection types. Submit only one.

 

Specimen Collection: Bone Marrow

Container(s): Green Top (Sodium Heparin)

Preferred volume to collect: 5.0 mL

Minimum volume to collect: 1.0 mL

Neonate volume to collect: 1.0 mL

Collection Instructions:

  • Collect bone marrow and transfer marrow from syringe to plain green top sodium heparin tube. 

 

Specimen Collection: Peripheral blood
Container/Tube: Green Top (Sodium Heparin)

Preferred volume to collect: 8.0 mL

Minimum volume to collect: 0.5 mL

Neonate volume to collect: 0.5 mL

Collection Instructions:

  • Peripheral blood may be substituted for Bone Marrow if circulating blasts are present 
  • Collect peripheral blood into a plain green top sodium heparin tube. 
  • Keep sample at room temperature and send to the Lab immediately. 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Spin: No

Aliquot: No

 

Transport Temperature: Ambient

If ambient temperature exceeds 80°F or is below 40°F, please send in an insulated container. All samples should be directed to the laboratory as soon as possible.

Specimen Stability

Ambient: 3 days (beyond 3 days, specimen viability will be detemined by Cytogenetics Lab)

Laboratory Retention: primary specimens 4 weeks; frozen pellets retained greater than 1 year

Test Frequency

Test Set Up: Monday – Friday 8:00 am – 5:00 pm, Saturday 8:00 am – 3:00 pm.

Technologist on call for Sunday set up.

Final report: 2 – 3 days.

Reference Range

An interpretive report will be provided.

Performing Department

Cytogenetics

Performing Department Laboratory Location

Corewell Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Performed by fluorescence in situ hybridization (FISH). This test was developed and its performance characteristics determined by Spectrum Health Cytogenetics Laboratory. It has not been cleared or approved by the US FDA. The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes.

CPT

88275 x6, 88271 x11

CDM Code

3108827102, 3108827501

Epic Test ID

1230101197

LOINC

Specimen Type: 66746-9

Indication Provided: 42349-1

Result: in process

Interpretation: 59050-5

Comments: 69965-2

Reviewed Date

4/28/2023