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Epic Code LAB255 Cytomegalovirus (CMV) Culture

Test Name Alias

Culture Virus CMV | 4234

Interface Order Alias

50021

Icons & Photos

Green top (Sodium Heparin) Sterile container with screw-on lid ITM-1072848
Universal Transport Media (UTM) Kit with Mini-Tip Swab for NP collection [ITM-1140078]  

NOTE: Urine collected in a gray top tube w/boric acid is an unacceptable specimen

Specimen Requirements

Submit only one of the following specimens:

Label specimen with at least 2 patient identifiers (usually name and date of birth)

 

Blood


Specimen Type: Blood
Container/Tube: Green top (Sodium Heparin)
Volume: 7 mL adults; 3 mL child

Minimum volume: 2 mL
Collection Instructions: Keep at room temperature, transport to laboratory immediately

 

Bronchoalveolar lavage (BAL) fluid or Sputum

Specimen Type: Bronchoalveolar lavage (BAL) fluid, Sputum

Container/Tube: Sterile collection container

 

 

NP Swab

Specimen Type:  Nasopharyngeal (NP) swab

Container/Tube: 3-mL UTM/Swab set (UTM medium with Regular Flocked Swab)

Collection Instructions: 

(Link) Nasopharyngeal Collection

  • Tilt the head of the patient, gently insert the swab through one of the nostrils and into the nasal passage.
  • Rotate the swab tip several times across the mucosal surface.
  • Remove the swab and insert into the dry tube.
  • Push the swab into the tube and break the plastic shaft swab at the break line.
  • Replace cap and screw on tightly. Ensure cap is secured! 
  • Label specimen with at least 2 patient identifiers (usually name and date of birth)
  • Place each specimen in the priority transport bag that came with the collection kit
  • Send to lab immediately 
  • Off site transportation temperature: Refrigerated specimen

 

Throat Swab or Eye Swab

Specimen Type: Throat swab, Eye swab
Container/Tube: 3-mL UTM/Swab set (UTM medium with Regular Flocked Swab)
Collection Instructions: Rotate the swab tip several times across the mucosal surface. Remove the swab and insert into the red top tube of the viral transport medium (UTM) immediately. Push the swab into the tube and break the plastic swab shaft at the break line. Replace cap and screw on tightly. Transport to lab immediately, refrigerate or on ice.

 

Body Fluid (indicate type/source), Amniotic Fluid, Breast Milk, Saliva, Stool or Urine

Specimen Type: Amniotic fluid, Breast Milk, Body Fluid (indicate type/source), Saliva, Stool, or Urine (clean catch specimen)
Container/Tube: Screw cap, sterile container

Minimum Volume: 1 mL
Collection Instructions: 

  • List containers separately.
  • Replace cap and screw on tightly.
  • Transport to lab immediately.
  • Urine collected in a gray top tube w/boric acid is an unacceptable specimen
  • Detailed Instructions (link): Urine Collection Guide for Staff

 

Tissue

Specimen Type: Tissue

Container/Tube: Sterile Container with screw-on lid. Viral Transport Media (UTM) may be used if immediate transport is not available.

Rejection Criteria

Urine collected in a gray top tube w/boric acid is an unacceptable specimen

Specimen Stability

Ambient: Not acceptable

Refrigerate: 24 hours

Frozen: Not acceptable

Laboratory Retention: The specimen is inoculated into UTM and stored frozen for 3 days.

Test Frequency

TAT 21 days, the test is performed daily on the first shift. The culture  report  remains in a preliminary status with updates at 24 hours and  4 days then  finalized at 21 days.

Reference Range

Negative for CMV

Performing Department

MICROBIOLOGY

Performing Department Laboratory Location

Spectrum Health Regional Laboratory, Grand Rapids, MI

Methodology

Cell culture

CPT

87252, 87254

CDM Code

4114234, 4118909

Epic Test ID

1230100573

Beaker LOINC

CYTOMEGALOVIRUS CULTURE: 5837-0

Reviewed Date

7/31/2021

Beaker Names

Beaker Procedure Name: CMV CULTURE
Beaker Display Name: Cytomegalovirus (CMV) Culture
BEAKER TEST NAME: CMV CULTURE
BEAKER TEST REPORT NAME: CMV Culture

Beaker Synonyms

No synonym on file

Beaker Location, Container and Temperature

BW MICROBIOLOGY: UNIVERSAL TRANSPORT MEDIUM R (Preferred)-Refrigerated
BW MICROBIOLOGY: 4 ML GREEN NA HEPARIN TUBE (Acceptable)-Ambient
BW MICROBIOLOGY: STERILE CONTAINER R (Acceptable)-Refrigerated