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Epic Code LAB274 Direct Coombs

Important Note

Specimens for Blood Bank must have 2 signatures on the specimen written in ink that is resistant to smearing. See Collection Information for more info.

Test Name Alias

Direct Coombs | DAT | Direct Antiglobulin Test | 8500

Interface Order Alias

60009

Icons & Photos

6 mL Pink Top (EDTA) 3 mL Lavender Top (EDTA)

Collection Instructions

Specimen Collection: Blood

 

Container(s): 6 mL Pink Top (EDTA) or 3 mL Lavender Top (EDTA)

Preferred Volume to Collect: 6.0 mL

Minimum Volume to Collect: 1.0 mL

Neonate Volume to Collect: 0.5 mL

Capillary collect ok? Yes

 

Collection Instructions:

  • Gently invert EDTA tube(s) 8 - 10 times after collection.
  • Specimens for Blood Bank must have 2 signatures on the specimen written in ink that is resistant to smearing:
    • User ID's or initials of collectors/witnesses
      • Outpatient collection: The patient may serve as witness and initial the tube label.
      • Policytech reference #6476
  • Specimen must be labeled in the presence of the patient with:
    • Patient's first and last name
    • Unique identification number (MRN)
    • Date of collection
  • If there is any question as to the validity of the specimen identification or an inconsistency between current and previous results, a new specimen must be collected.

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Processed Specimen: Whole Blood EDTA

Centrifuge/Spin: No

Aliquot: No

Transport Temperature: Ambient or Refrigerate

Specimen Stability

Ambient: 24 hours

Refrigerate: 7 days

Laboratory Retention: 7 days

Reflex Information

Link: Reflex approved by MEC

Test Frequency

Available 24 hours, usual TAT 1 day.

Reference Range

Negative

Performing Department

BLOOD BANK

Performing Department Laboratory Location

Spectrum Health Big Rapids Laboratory, Big Rapids, MI
Spectrum Health Blodgett Laboratory, Grand Rapids, MI
Spectrum Health Butterworth Laboratory. Grand Rapids, MI
Spectrum Health Gerber Laboratory, Fremont, MI
Spectrum Health Kelsey Laboratory, Lakeview, MI
Spectrum Health Ludington Laboratory, Ludington, MI
Spectrum Health Pennock Laboratory, Hastings, MI
Spectrum Health Reed City Laboratory, Reed City, MI
Spectrum Health United Laboratory, Greenville, MI
Spectrum Health Zeeland Laboratory, Zeeland, MI

Methodology

Tube technique

CPT

 86880-LAB-ANTIGLOBULIN DIRECT

CDM Code

4108500

Epic Test ID

1230100612

LOINC

Poly antisera: 1007-4

IgG antisera: 55776-9

C3 antisera: 56471-6

Beaker LOINC

LOINC Not available

Reviewed Date

1/29/2020

Beaker Names

Beaker Procedure Name: DIRECT ANTIGLOBULIN TEST
Beaker Display Name: Direct Antiglobulin Test
BEAKER TEST NAME: DIRECT COOMBS
BEAKER TEST REPORT NAME: Direct Coombs

Beaker Synonyms

DAT|DIRECT COOMBS

Beaker Collection and Specimen Handling

BEAKER COLLECTION: BB 2 SIGNERS

Beaker Location, Container and Temperature

BW BLOOD BANK: 6 ML PINK EDTA TUBE A (Preferred)-Ambient
BW BLOOD BANK: 3 ML LAV TUBE R (Acceptable)-Refrigerated
BW BLOOD BANK: 3 ML LAVENDER TOP TUBE A (Acceptable)-Ambient
BW BLOOD BANK: 6 ML PINK EDTA R (Acceptable)-Refrigerated
BW BLOOD BANK: LAVENDER (EDTA) MICROTAINER (Acceptable)-No Temperature
BL BLOOD BANK: 6 ML PINK EDTA TUBE A (Preferred)-Ambient
BL BLOOD BANK: 3 ML LAV TUBE R (Acceptable)-Refrigerated
BL BLOOD BANK: 3 ML LAVENDER TOP TUBE A (Acceptable)-Ambient
BL BLOOD BANK: 6 ML PINK EDTA R (Acceptable)-Refrigerated
BL BLOOD BANK: LAVENDER (EDTA) MICROTAINER (Acceptable)-No Temperature
BR BLOOD BANK: 6 ML PINK EDTA TUBE A (Preferred)-Ambient
BR BLOOD BANK: 3 ML LAV TUBE R (Acceptable)-Refrigerated
BR BLOOD BANK: 3 ML LAVENDER TOP TUBE A (Acceptable)-Ambient
BR BLOOD BANK: 6 ML PINK EDTA R (Acceptable)-Refrigerated
BR BLOOD BANK: LAVENDER (EDTA) MICROTAINER (Acceptable)-No Temperature
GM BLOOD BANK: 6 ML PINK EDTA TUBE A (Preferred)-Ambient
GM BLOOD BANK: 3 ML LAV TUBE R (Acceptable)-Refrigerated
GM BLOOD BANK: 3 ML LAVENDER TOP TUBE A (Acceptable)-Ambient
GM BLOOD BANK: 6 ML PINK EDTA R (Acceptable)-Refrigerated
GM BLOOD BANK: LAVENDER (EDTA) MICROTAINER (Acceptable)-No Temperature
KL BLOOD BANK: 6 ML PINK EDTA TUBE A (Preferred)-Ambient
KL BLOOD BANK: 3 ML LAV TUBE R (Acceptable)-Refrigerated
KL BLOOD BANK: 3 ML LAVENDER TOP TUBE A (Acceptable)-Ambient
KL BLOOD BANK: 6 ML PINK EDTA R (Acceptable)-Refrigerated
KL BLOOD BANK: LAVENDER (EDTA) MICROTAINER (Acceptable)-No Temperature
LH BLOOD BANK: 6 ML PINK EDTA TUBE A (Preferred)-Ambient
LH BLOOD BANK: 3 ML LAV TUBE R (Acceptable)-Refrigerated
LH BLOOD BANK: 3 ML LAVENDER TOP TUBE A (Acceptable)-Ambient
LH BLOOD BANK: 6 ML PINK EDTA R (Acceptable)-Refrigerated
LH BLOOD BANK: LAVENDER (EDTA) MICROTAINER (Acceptable)-No Temperature
PH BLOOD BANK: 6 ML PINK EDTA TUBE A (Preferred)-Ambient
PH BLOOD BANK: 3 ML LAV TUBE R (Acceptable)-Refrigerated
PH BLOOD BANK: 3 ML LAVENDER TOP TUBE A (Acceptable)-Ambient
PH BLOOD BANK: 6 ML PINK EDTA R (Acceptable)-Refrigerated
PH BLOOD BANK: LAVENDER (EDTA) MICROTAINER (Acceptable)-No Temperature
RC BLOOD BANK: 6 ML PINK EDTA TUBE A (Preferred)-Ambient
RC BLOOD BANK: 3 ML LAV TUBE R (Acceptable)-Refrigerated
RC BLOOD BANK: 3 ML LAVENDER TOP TUBE A (Acceptable)-Ambient
RC BLOOD BANK: 6 ML PINK EDTA R (Acceptable)-Refrigerated
RC BLOOD BANK: LAVENDER (EDTA) MICROTAINER (Acceptable)-No Temperature
UN BLOOD BANK: 6 ML PINK EDTA TUBE A (Preferred)-Ambient
UN BLOOD BANK: 3 ML LAV TUBE R (Acceptable)-Refrigerated
UN BLOOD BANK: 3 ML LAVENDER TOP TUBE A (Acceptable)-Ambient
UN BLOOD BANK: 6 ML PINK EDTA R (Acceptable)-Refrigerated
UN BLOOD BANK: LAVENDER (EDTA) MICROTAINER (Acceptable)-No Temperature
ZH BLOOD BANK: 6 ML PINK EDTA TUBE A (Preferred)-Ambient
ZH BLOOD BANK: 3 ML LAV TUBE R (Acceptable)-Refrigerated
ZH BLOOD BANK: 3 ML LAVENDER TOP TUBE A (Acceptable)-Ambient
ZH BLOOD BANK: 6 ML PINK EDTA R (Acceptable)-Refrigerated
ZH BLOOD BANK: LAVENDER (EDTA) MICROTAINER (Acceptable)-No Temperature