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HelpTest Code LAB293 Complete Blood Count (CBC) w/ Differential
Test Name Alias
CBC w/ Diff | CBCD | 8411
Collection Instructions
Specimen Collection: Blood
Container(s): Lavender top (EDTA)
Preferred Volume to Collect: 3.0 mL
Minimum Volume to Collect: 0.5 mL
Neonate Volume to Collect: 0.5 mL
Capillary collect ok? Yes
Microtainer acceptable: Yes
Collection Instructions:
- After collection, gently invert tube 8-10 times.
- Specimen must be received within 6 hours of collection if ambient, 10 hours if refrigerated (see stability information below)
Processing Instructions (Laboratory, Outpatient or Off-site collection)
Processed Specimen: Whole Blood EDTA
Centrifuge/Spin: No
Aliquot: No
Transport Temperature: Refrigerate (preferred) or Ambient (acceptable)
Specimen Stability
Ambient: 6 hours
Refrigerate: (Preferred) 10 hours
Note: Cellular characteristics required for differential analysis become compromised in specimens greater than 10 hours due to degradation of cells
Laboratory Retention: 72 hours
United Laboratory:
Ambient: 24 hours
Refrigerate: 36 hours
Laboratory Retention: 7 days
Reflex Information
Review document (link): Reflex Testing Offered
Test Frequency
Available 24 hours, usual TAT 1 day.
Reference Range
Baso Absolute
0 – 0.2 ( x 103/uL)
Basophil Auto
0 – 2 %
Eos Absolute
0 – 0.5 ( x 103/uL)
Eos Auto
0 – 6 %
Hct
Male/Female
0 Minutes – 1 Months: 42 – 64 %
1 Months – 3 Months: 38 – 56 %
3 Months – 1 Years: 33 – 48 %
1 Years – 3 Years: 32 – 44 %
3 Years – 9 Years: 35 – 45 %
9 Years – 13 Years: 37 – 47 %
Male
13 Years – 150 Years: 42 – 52 %
Female
13 Years – 150 Years: 37 – 47 %
Hgb
Male/Female
0 Minutes – 1 Months: 15.5 – 21 g/dL
1 Months – 3 Months: 12.5 – 19 g/dL
3 Months – 1 Years: 10.5 – 16 g/dL
1 Years – 3 Years: 10 – 14 g/dL
3 Years – 9 Years: 11.5 – 14.5 g/dL
Male
9 Years – 13 Years: 12.5 – 16 g/dL
13 Years – 150 Years: 14 – 18 g/dL
Female
9 Years – 13 Years: 12 – 15 g/dL
13 Years – 150 Years: 12 – 16 g/dL
IG %
0.1 - 0.6 %
IG Absolute
0.01 to 0.05 x 103/uL
Lymph Absolute
Male/Female
0 Minutes – 1 Months: 2.5 – 10 ( x
103/uL)
1 Months – 3 Years: 2.5 – 12 ( x
103/uL)
3 Years – 9 Years: 1.8 – 8 ( x
103/uL)
9 Years – 13 Years: 1.4 – 6 ( x
103/uL)
13 Years – 150 Years: 1 – 4 ( x
103/uL)
Lymph Auto
Male/Female
0 Minutes – 1 Months: 30 – 66 %
1 Months – 3 Years: 42 – 75 %
3 Years – 9 Years: 30 – 66 %
9 Years – 13 Years: 25 – 50 %
13 Years – 150 Years: 20 – 50 %
MCH
Male/Female
0 Minutes – 1 Months: 32 – 41 pg
1 Months – 3 Months: 30 – 38 pg
3 Months – 1 Years: 27 – 35 pg
1 Years – 3 Years: 23 – 30 pg
3 Years – 9 Years: 25 – 32 pg
9 Years – 150 Years: 27 – 33 pg
MCHC
Male/Female
0 Minutes – 1 Months: 35 – 38 g/dL
1 Months – 3 Months: 34 – 37 g/dL
3 Months – 1 Years: 33 – 37 g/dL
1 Years – 150 Years: 32 – 37 g/dL
MCV
Male/Female
0 Minutes – 1 Months: 94 – 116 fL
1 Months – 3 Months: 84 – 104 fL
3 Months – 3 Years: 74 – 90 fL
3 Years – 9 Years: 80 – 94 fL
9 Years – 13 Years: 84 – 98 fL
13 Years – 150 Years: 80 – 100 fL
Mono Absolute
0 – 0.9 ( x 103/uL)
Mono Auto
2 – 12 %
MPV
7.4 – 11 fL
Neut Absolute
Male/Female
0 Minutes – 1 Months: 2 – 10 ( x
103/uL)
1 Months – 13 Years: 1.5 – 6 ( x
103/uL)
13 Years – 150 Years: 1.8 – 7.8 ( x
103/uL)
Neut Auto
Male/Female
0 Minutes – 1 Months: 25 – 64 %
1 Months – 3 Years: 19 – 45 %
3 Years – 9 Years: 30 – 64 %
9 Years – 13 Years: 35 – 70 %
13 Years – 150 Years: 35 – 80 %
NRBC Absolute
0.00 - 0.01 x 103/uL
NRBC %
0.0 - 0.1%
Platelet
140 – 400 ( x 103/uL)
RBC
Male
0 Minutes – 1 Months: 4.6 – 6.2 ( x
106/uL)
1 Months – 3 Months: 4.4 – 5.8 ( x
106/uL)
3 Months – 13 Years: 4.2 – 5.2 ( x
106/uL)
13 Years – 150 Years: 4.6 – 6 ( x
106/uL)
Female
0 Minutes – 1 Months: 4.6 – 6.2 ( x
106/uL)
1 Months – 3 Months: 4.4 – 5.8 ( x
106/uL)
3 Months – 13 Years: 4.2 – 5.2 ( x
106/uL)
13 Years – 150 Years: 4.2 – 5.4 ( x
106/uL)
RDW
11 – 16 %
WBC
Male/Female
0 Minutes – 1 Months: 6 – 20 ( x 103/uL)
1 Months – 3 Years: 6 – 18 ( x
103/uL)
3 Years – 9 Years: 5 – 15 ( x
103/uL)
9 Years – 13 Years: 4.5 – 12 ( x
103/uL)
13 Years – 150 Years: 4 – 10.8 ( x
103/uL)
Performing Department
HEMATOLOGY
Performing Department Laboratory Location
Spectrum Health Regional Laboratory, Grand Rapids, MI
Spectrum Health Big Rapids Laboratory, Big Rapids, MI
Spectrum Health Blodgett Laboratory, Grand Rapids, MI
Spectrum Health Gerber Laboratory, Fremont, MI
Spectrum Health Kelsey Laboratory, Lakeview, MI
Spectrum Health Ludington Laboratory, Ludington, MI
Spectrum Health Pennock Laboratory, Hastings, MI
Spectrum Health Reed City Laboratory, Reed City, MI
Spectrum Health United Laboratory, Greenville, MI
Spectrum Health Zeeland Laboratory, Zeeland, MI
Methodology
The Sysmx analyzer counts and sizes red blood cells (RBC) and platelets (PLT) using electronic resistance detection enhanced by hydrodynamic focusing. Hematocrit (HCT) is measured as the ratio of the total RBC volume to whole blood using cumulative pulse height detection. Hemoglobin (HGB) is converted to SLS-hemoglobin and read photometrically.
WBC count, differential, reticulocytes (RET) and nucleated red blood cells (NRBC) are all evaluated using flow cytometry with a semiconductor laser exploiting the differences in cell size, complexity and RNA/DNA content. WBC and basophils (BASO) are treated with an acidic lyse that lyses RBC and WBC, but not BASO. The remaining WBC nuclei and intact BASO are differentiated by cell size and internal cell structure. The WBC differential channel classifies lymphocytes (LYMPH), monocytes (MONO), eosinophils (EO), granulocytes, and immature granulocytes (IG) by cellular complexity and nucleic acid content. The differential cell placement is then enhanced using Adaptive Cluster Analysis. Reticulocytes are separated from mature RBC and PLT by size and RNA content. NRBC are separated from WBC based on nuclear size after lysing and DNA/RNA staining.
The Immature Information channel (IMI) cytochemically differentiates immature myeloid cells from mature granulocytes based on the membrane lipid content, and direct current and radio frequency technologies.
CPT
85025
85055- IPF Reflex
85027
85007-Reflex Differential
CDM Code
4068410
Epic Test ID
1230101458
Interface Order Alias
11308
Reviewed Date
3/30/2018