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Test Code LAB294 Complete Blood Count (CBC) w/o Differential

Test Name Alias

CBC w/o Diff | 8397

Collection Instructions

Specimen Collection: Blood

 

Container(s): Lavender top (EDTA)

Preferred Volume to Collect: 3.0 mL

Minimum Volume to Collect: 0.5 mL

Neonate Minimum Volume to Collect: 0.25 mL

Capillary collect ok? Yes

Microtainer acceptable: Yes

 

Collection Instructions:

  • Gently invert EDTA tube(s) 7 – 8 times after collection.
  • Specimen must be received within 6 hours of collection. 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Processed Specimen: Whole Blood EDTA

 

Centrifuge/Spin: No

Aliquot: No

 

Transport Temperature: Refrigerate (preferred) or Ambient (acceptable)

Specimen Transport Temperature

Refrigerated (preferred)

Ambient (acceptable)

Specimen Stability

Ambient: 12 hours

Refrigerate: 24 hours

Laboratory Retention: 72 hours

United Laboratory Retention: 7 days

Test Frequency

Available 24 hours, usual TAT 1 day.

Reference Range

Hematocrit (Hct)

0 Minutes up to 1 Month: 42.0 – 64.0 %
1 Month up to 3 Months: 38.0 – 56.0 %
3 Months up to 1 Year: 33.0 – 48.0 %
1 Year up to 3 Years: 32.0 – 44.0 %
3 Years up to 9 Years: 35.0 – 45.0 %
9 Years up to 13 Years: 37.0 – 47.0 %

Male

13 Years up to 150 Years: 42.0 – 52.0 %

Female

13 Years up to 150 Years: 37.0 – 47.0 %

 

Hemoglobin (Hgb)

0 Minutes up to 1 Month: 15.5 – 21.0 g/dL
1 Month up to 3 Months: 12.5 – 19.0 g/dL
3 Months up to 1 Year: 10.5 – 16.0 g/dL
1 Year up to 3 Years: 10.0 – 14.0 g/dL
3 Years up to 9 Years: 11.5 – 14.5 g.dL

Male

9 Years up to 13 Years: 12.5 – 16.0 g/dL
13 Years up to 150 Years: 14.0 – 18.0 g/dL

Female

9 Years up to 13 Years: 12.0 – 15.0 g/dL
13 Years up to 150 Years: 12.0 – 16.0 g/dL

 

Mean Cell Hemoglobin (MCH)

0 Minutes up to 1 Month: 32.0 – 41.0 pg
1 Month up to 3 Months: 30.0 – 38.0 pg
3 Months up to 1 Year: 27.0 – 35.0 pg
1 Year up to 3 Years: 23.0 – 30.0 pg
3 Years up to 9 Years: 25.0 – 32.0 pg
9 Years up to 150 Years: 27.0 – 33.0 pg

 

Mean Cell Hemoglobin Concentration (MCHC)

0 Minutes up to 1 Month: 35.0 – 38.0 g/dL
1 Month up to 3 Months: 34.0 – 37.0 g/dL
3 Months up to 1 Year: 33.0 – 37.0 g/dL
1 Year up to 150 Years: 32.0 – 37.0 g/dL

 

Mean Cell Volume (MCV)

0 Minutes up to 1 Month: 94.0 – 116.0 fL

1 Month up to 3 Months: 84.0 – 104.0 fL

3 Months up to 3 Years: 74.0 – 90.0 fL

3 Years up to 9 Years: 80.0 – 94.0 fL

9 Years up to 13 Years: 84.0 – 98.0 fL

13 Years up to 150 Years: 80.0 – 100.0 fL

 

Mean Platelet Volume (MPV)

7.4 – 11.0 fL

 

Nucleated Red Blood Cell (NRBC) Absolute Count

0.00 - 0.01 x 103/uL

 

Nucleated Red Blood Cell (NRBC) Automated

0.0 - 0.1%

 

Platelet

140 – 400  ( x 103/uL)

 

Red Blood Cell (RBC)

Male

0 Minutes up to 1 Month: 4.60 – 6.20 ( x 106/uL)

1 Month up to 3 Months: 4.40 – 5.80  ( x 106/uL)

3 Months up to 13 Years: 4.20 – 5.20  ( x 106/uL)

13 Years up to 150 Years: 4.60 – 6.00  ( x 106/uL)

 

Female

0 Minutes up to 1 Month: 4.60 – 6.20  ( x 106/uL)

1 Month up to 3 Months: 4.40 – 5.80  ( x 106/uL)

3 Months up to 13 Years: 4.20 – 5.20  ( x 106/uL)

13 Years up to 150 Years: 4.20 – 5.40  ( x 106/uL)

 

Red Cell Distribution Width (RDW)

11.0 – 16.0 %

 

White Blood Cell (WBC)

0 Minutes up to 1 Month: 6.00 – 20.00 ( x 103/uL)

1 Month up to 3 Years: 6.00 – 18.00 ( x 103/uL)

3 Years up to 9 Years: 5.00 – 15.00 ( x 103/uL)

9 Years up to 13 Years: 4.50 – 12.00 ( x 103/uL)

13 Years up to 150 Years: 4.00 – 10.80 ( x 103/uL)

 

The pediatric data is summarized/complied from the literature.

 

Laboratory Critical Values Table

Performing Department

HEMATOLOGY

Performing Department Laboratory Location

Spectrum Health Regional Laboratory, Grand Rapids, MI
Spectrum Health Big Rapids Laboratory, Big Rapids, MI
Spectrum Health Blodgett Laboratory, Grand Rapids, MI
Spectrum Health Gerber Laboratory, Fremont, MI
Spectrum Health Kelsey Laboratory, Lakeview, MI
Spectrum Health Ludington Laboratory, Ludington, MI
Spectrum Health Pennock Laboratory, Hastings, MI
Spectrum Health Reed City Laboratory, Reed City, MI
Spectrum Health United Laboratory, Greenville, MI
Spectrum Health Zeeland Laboratory, Zeeland, MI

Methodology

The Sysmex analyzer counts and sizes red blood cells (RBC) and platelets (PLT) using electronic resistance detection enhanced by hydrodynamic focusing. Hematocrit (HCT) is measured as the ratio of the total RBC volume to whole blood using cumulative pulse height detection. Hemoglobin (HGB) is converted to SLS-hemoglobin and red photometrically.

 

WBC count, differential, reticulocytes (RET) and nucleated red blood cells (NRBC) are all evaluated using flow cytometry with a semiconductor laser exploiting the differences in cell size, complexity and RNA/DNA content. WBC and basophils (BASO) are treated with an acidic lyse that lyses RBC and WBC, but not BASO. The remaining WBC nuclei and intact BASO are differentiated by cell size and internal cell structure.

CPT

85027

85055- IPF Reflex

CDM Code

4068397

Epic Test ID

1230100453

Interface Order Alias

10033

LOINC

WBC: 6690-2

RBC: 789-8

Hgb: 718-7

Hct: 4544-3

MCV: 787-2

MCH: 785-6

MCHC: 786-4

RDW: 788-0

Platelet: 777-3

MPV: 32623-1

Reviewed Date

3/30/2018