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HelpEpic Code LAB3079 CD20 and CD19
Test Name Alias
CD20 (CD20, CD19) | Rituxan Therapy Test | B-Cells | CD20 by Flow Cytometry | 4060
Interface Order Alias
11293
Quick Collect
+A
Clinical Information
The CD20 flow cytometry assay quantitatively measures therapeutic B-cell depletion for patients on monoclonal anti-CD20 therapy such as Rituximab. Anti-CD20 therapies are used to treat patients with various types of B cell lymphoma, leukemia, and immune disorders. WBCs are stained with fluorescently labeled monoclonal antibodies to CD14, CD19, CD20, and CD45 and run on a flow cytometer and the CD19 and CD20 antigen expression are analyzed. The percentages and absolute counts of CD19 and CD20 are included in the patient's report.
Collection Instructions
Specimen Collection: Blood
Container(s): Lavender top (EDTA)
Preferred Volume to Collect: 3.0 mL
Minimum Volume to Collect: 1.0 mL
Neonate Volume to Collect: 0.5 mL
Capillary collect ok? No
Microtainer acceptable? Yes
Collection Instructions:
- Gently invert EDTA tube(s) 8 - 10 times after collection.
- Transport as soon as possible
Processing Instructions (Laboratory, Outpatient or Off-site collection)
Processed Specimen: Whole Blood EDTA
Centrifuge/Spin: No
Aliquot: No
Transport Temperature: Ambient (transport as soon as possible)
Rejection Criteria
Specimens that do not meet Spectrum Health Laboratory standards may be rejected (cancelled) due to:
- Refrigerated specimen
- Clotted
- Grossly hemolyzed
Specimen Stability
Ambient: 48 hours
Note: Specimen must remain ambient as all times and testing must be performed within 48 hours of collection
Test Frequency
Available Monday – Friday, usual TAT 2 days
Reference Range
Normal Lymphocyte Subset Percentages of Peripheral Blood Lymphocytes
| 0-3 mos | 3-6 mos | 6-12 mos | 1-2 yrs | 2-6 yrs | 6-12 yrs | 12-18 yrs | 18+ yrs | |
| CD3% | 53-84 | 51-77 | 49-76 | 53-75 | 56-75 | 60-76 | 56-84 | 56-86 |
| CD19% | 6-32 | 11-41 | 14-37 | 16-35 | 16-35 | 13-27 | 6-23 | 5-22 |
| CD16/56% | 4-18 | 3-14 | 3-15 | 3-15 | 3-15 | 4-17 | 3-22 | 5-26 |
| CD3/CD4% | 35-64 | 35-56 | 31-56 | 32-51 | 32-51 | 31-47 | 31-52 | 33-58 |
| CD3/CD8% | 12-28 | 12-23 | 12-24 | 14-30 | 14-30 | 18-35 | 18-35 | 13-39 |
| CD4/CD8 ratio | 0.9-2.9 | 0.9-2.9 | 0.9-2.9 | 0.9-2.9 | 0.9-2.9 | 0.9-2.9 | 0.9-2.9 | 0.9-2.9 |
Normal Lymphocyte Subset Absolute Counts of Peripheral Blood Lymphocytes (cells/µL)
| 0-3 mo | 3-6 mo | 6-12 mo | 1-2 yr | 2-6 yr | 6-12 yr | 12-18 yr | 18+ yrs | |
| CD3 | 2500-5500 | 2500-5600 | 1900-5900 | 2100-6200 | 1400-3700 | 1200-2600 | 1000-2200 | 723-2764 |
| CD19 | 300-2000 | 430-3000 | 610-2600 | 720-2600 | 390-1400 | 270-860 | 110-570 | 80-616 |
| CD16/56 | 170-1100 | 170-830 | 160-950 | 180-920 | 130-720 | 100-480 | 70-480 | 84-724 |
| CD3/CD4 | 1600-4000 | 1800-4000 | 1400-4300 | 1300-3400 | 700-2200 | 650-1500 | 530-1300 | 404-1612 |
| CD3/CD8 | 560-1700 | 590-1600 | 500-1700 | 620-2000 | 490-1300 | 370-1100 | 330-920 | 220-1129 |
Reference:
Lymphocyte subsets in healthy children from birth through 18 years of age:
The Pediatric AIDS Clinical Trials Group P1009 study
Performing Department
Flow Cytometry
Performing Department Laboratory Location
Corewell Health Advanced Technology Laboratory (ATL), Grand Rapids, MI
Methodology
Flow Cytometry
CPT
86356, 86355
CDM Code
3008635603, 3028635501
Epic Test ID
1230100455
LOINC
White Cell Count: 6690-2
Lymphs from Auto Diff %: N/A
Lymphs from Auto Diff Count: N/A
CD19 Total B Lymphocytes %: 8117-4
CD19 Total B Lymphocytes Cell Count: 8116-6
CD20 B Lymphocytes %: 8119-0
CD20 B Lymphocytes Cell Count: 9558-8
Mayo Access Code
SHO4060
Reviewed Date
4/28/2023