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Epic Code LAB3275 MDM2 Amplification by FISH

Test Name Alias

MDM2 by FISH | 9225

Interface Order Alias

11606

Quick Collect

AP Ambient

Clinical Information

Fluorescence in situ hybridization (FISH) using MDM2 DNA probe aides in the differential diagnosis between well-differentiated liposarcoma and benign lipoma.

Collection Instructions

Multiple specimen collection types. Submit only one.

 

Specimen Collection: Formalin-fixed, paraffin-embedded (FFPE) tissue

Container(s): Block

Preferred Volume to Collect: 4 unstained slides cut between 4 and 6 microns thick

Collection Instructions:

  • Include an H&E slide scribed by Pathologist and Surgical Specimen Testing order sheet.
  • Place specimen in 10% Formalin and order Tissue Pathology in addition to MDM2 Amplification by FISH order.

 

Specimen Collection: Fine Needle Aspirate (FNA, such as lymph node)

Collection Instructions: Order FNA Cytology (refer to test code 202 for collection instructions) in addition to MDM2 Amplification by FISH order.

 

 

Specimen Collection:  Pleural Fluid

Collection instructions:  Place specimen in sterile container and order Medical Cytology in addition to MDM2 Amplification by FISH order.

 

 

Specimen Collection: Fresh Tumor: 1.0 cm³ biopsy specimen under sterile conditions.

Container(s): Sterile container

Collection Instructions: 

  • Place in sterile container with either tissue culture media or transport media and an antibiotic such as Pen/Strep or Gentamicin.
  • Tissue may be stored overnight in medium at 4° C.

 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Spin: No

Aliquot: No

 

Transport Temperature: Ambient (Fresh Tumor, should be refrigerated temperature)

If ambient temperature exceeds 80°F or is below 40°F, please send in an insulated container. All samples should be directed to the laboratory as soon as possible.

Specimen Stability

Ambient: 3 days (beyond 3 days, specimen viability will be detemined by Cytogenetics Lab)

Laboratory Retention: primary specimens 4 weeks; frozen pellets retained greater than 1 year

Test Frequency

Test Set Up: Monday – Friday 8:00 am – 5:00 pm, Saturday 8:00 am – 3:00 pm.

Technologist on call for Sunday set up.

Final report: 2-7 days.

Reference Range

An interpretative report will be provided.

Performing Department

Cytogenetics

Performing Department Laboratory Location

Corewell Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Performed by fluorescence in situ hybridization (FISH). This test was developed and its performance characteristics determined by Spectrum Health Cytogenetics Laboratory. It has not been cleared or approved by the US FDA. The FDA has determined that such clearance is not necessary. This test is used for clinical purposes.

CPT

88275, 88271 x2

CDM Code

3108827102, 3108827401

Epic Test ID

1230100919

LOINC

Specimen Type: 66746-9

Indication Provided: 42349-1

Result: in process

Interpretation: 59050-5

Comments: 69965-2

Reviewed Date

4/28/2023