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Epic Code LAB3326 VerifyNow® P2Y12 assay for Antiplatelet Effect

Important Note

5/28/21 There is currently a shortage of this tube. Please contact Phlebotomy Services for tube availability prior to ordering the VerifyNow Assay.

Due to the special handling and time sensitivity, this test will ONLY be drawn on

INPATIENTS: 

 

OUTPATIENTS: 

This test has not been validated for transport and cannot be couriered from offsite locations.

 

Direct Venipuncture Reminder: Clearing tube must be drawn prior to drawing the TWO required Greiner vacuette tubes which must be labeled and sent together. Clearing tube does not have to be sent to the lab. See Indwelling Catheter section below.

Test Name Alias

Verify Now P2Y12 | Clopidogrel | 152

Interface Order Alias

11641

Ordering Instructions

This is a test for P2Y12 inhibition by clopidogrel (Plavix), prasugel (Effient), or ticlopidine (Ticlid).  Direct IIb/IIIa inhibitors will interfere. In addition the P2Y12 test must not be performed within 48 hours after administration of eptifibatide (Integrilin) or tirofibrin (Aggrastat), or within 14 days after administration of abciximab (ReoPro)

Collection Instructions

Direct Venipuncture

Specimen Type: Whole blood
Container/Tube: 2 Greiner Bio-One partial-fill vacuette tubes with 3.2% sodium citrate (blue top). Greiner #454322 + clearing tube
Volume: 2.0 mL each
Collection Instructions: 

  • Collect all tubes of whole blood using a 21 gauge or larger needle.
    • Butterfly (21 gauge) is OK to use. 
  • First collect a clearing tube (at least 2 mL) making sure the clearing tube does not contain any platelet inhibiting substances (e.g. EDTA).
    • Clearing tube does not need to be sent to lab
  • Fill the second and third tube (sample tubes) to the black line (1/2 tube).
    • Do not under fill.
  • If drawing blood for a CBC at the same time, fill the CBC tube last.
  • Gently invert the Greiner tubes at least 5 times to ensure complete mixing of the contents.
  • Samples with evidence of clotting should not be used.
  • Do not shake, as that may give incorrect results.
  • Label the tubes with the patient ID, date and time it was drawn.
  • Do not refrigerate.
  • Send both Greiner tubes!

 

Indwelling Catheter

Specimen Type: Whole blood
Container/Tube: 1 Greiner Bio-One partial-fill vacuette tube with 3.2% sodium citrate (blue top). Greiner #454322
Volume: 2.0 mL
Collection Instructions:

  • Discard the first 5 mL from an indwelling catheter to clear the line.
  • Ensure the catheter is free of clots.
  • Immediately transfer blood to a 2 mL Greiner Bio-One partial fill vacuette tube with 3.2% sodium citrate (blue top). Greiner #454322.
  • Fill both tubes to the black line (1/2 tube).
    • Do not under fill.
  • If drawing blood for a CBC at the same time, fill the CBC tube last.
  • Gently invert the Greiner tubes at least 5 times to ensure complete mixing of the contents.
  • Samples with evidence of clotting should not be used.
  • Do not shake as that may give incorrect results.
  • Label the tubes with the patient ID, date and time it was drawn.
  • Do not refrigerate.
  • Send both Greiner tubes!

 

Please see Resources field to the left for more information.

 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

This test has not been validated for transport and cannot be couriered from offsite locations.

 

Rejection Criteria

Hemolysis

Specimen Stability

Ambient: 4 hours

Refrigerate: Not Acceptable

Frozen: Not Acceptable

Laboratory Retention: 24 hours

Test Frequency

Available 24 hours, usual TAT 1 day.

Reference Range

Interpretive Comments

Verify Now P2Y12 assay for effect of clopidogrel (Plavix) and related drugs on platelet activity.

Verify Now P2Y12 assay results are reported in Platelet REactivity Unites (PRU)

Lower PRU values are indicative of effective antiplatelet drug inhibition of platelet activity.

Higher PRU values are indicative of ineffective antiplatelet drug inhibition of platelet activity.

Patients on chronic clopidogrel (Plavix) thearapy with PRU values at or below 180 are considered adequate responders.

Patients with PRU values at or above 208 may proceed to cardiac surgery.

Patient with PRU values below 208 should have Verify Now P2Y12 repeated in 12 - 24 hours.

Performing Department

COAGULATION

Performing Department Laboratory Location

Spectrum Health Regional Laboratory, Grand Rapids, MI

Spectrum Health Big Rapids Laboratory, Big Rapids, MI

Methodology

Point of Care (POC) utilizing platelet induced aggregation turbimetric optical detection

CPT

85576

CDM Code

4098608

Epic Test ID

1230101281

LOINC

Verify Now P2Y12: 49010-2

Beaker LOINC

LOINC Not available

Reviewed Date

10/11/2021

Beaker Names

Beaker Procedure Name: VERIFY NOW P2Y12
Beaker Display Name: Verify Now P2Y12 assay for Antiplatelet Effect
BEAKER TEST NAME: VERIFY NOW P2Y12
BEAKER TEST REPORT NAME: Verify Now P2Y12 assay for Antiplatelet Effect

Beaker Synonyms

No synonym on file

Beaker Collection and Specimen Handling

BEAKER COLLECTION: LOOK+LOOK

Beaker Location, Container and Temperature

BW COAGULATION: BLUE TOP GREINER BIO-ONE (Preferred)-Ambient
BR COAGULATION: BLUE TOP GREINER BIO-ONE (Preferred)-No Temperature