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Epic Code LAB3377 APTT Heparin Neutralization

Important Note

There is currently a nationwide shortage of this tube. Please take steps to ensure specimen integrity to reduce recollection. Specimen Tube Shortage (link)

Specimen tube must be full. The ratio of blood to anticoagulant is critical. Increased or decreased volumes of anticoagulant will cause erroneous results.

Test Name Alias

Activated Partial Thromboplastin Time (APTT) | APTT Heparin Neutralization

Interface Order Alias

11684

Clinical Information

For use as a heparin neutralizer in plasma to rule out heparin contamination in coagulation testing.

Panel Information

APTT

Heparin Neutralized PTT

Icons & Photos

Collection Instructions

Specimen Collection: Blood

 

Container(s): 3.2% Na. Citrate (blue tube)

Preferred Volume to Collect: 2.7 mL (full tube)

Minimum Volume to Collect: 1.8 mL (full tube)

Capillary collect ok: No

Microtainer acceptable: No

 

Collection Instructions:

  • Detailed instructions (link): Anticoagulant Correction for High Hematocrit

  • Detailed collection instructions (link): Blue Citrate Tube

  • Specimen should be processed within 4 hours – See Processing Instructions.

    • Specimen collected from patients receiving heparin therapy must be centrifuged within 1 hour --See Processing Instructions 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Processed Specimen: Platelet Poor Plasma

 

Centrifuge/Spin: Yes

Aliquot: Yes

 

Processing Instructions:

  • Detailed instructions (link): Preparing Platelet Poor Plasma
  • Separate plasma into 2 - 3 aliquots, minimum of 0.5 mL each.
  • Freeze aliquots in plastic transport tubes immediately.

Transport Temperature:

  • Ambient (Up to 1 hour in whole blood state)
  • Frozen (Plasma Only >1 hour post collection)

Rejection Criteria

  • Hemolysis
  • Specimen has been thawed

Specimen Stability

Ambient: 1 hour in whole blood state

Refrigerate: not acceptable

Frozen: Plasma only > 1 hour post collection

Laboratory Retention: 24 hours

Test Frequency

Available 24 hours, usual TAT 1 day.

Reference Range

  • 21 - 27 seconds (Spectrum Health Regional Lab, Grand Rapids - BCS XP analyzer)
  • 21 - 31 seconds (Spectrum Health Regional Lab, Grand Rapids - CS 5100 analyzer)

Performing Department

COAGULATION

Performing Department Laboratory Location

Spectrum Health Regional Laboratory, Grand Rapids, MI

Methodology

Heparinase cleaves heparin at multiple sites per molecule.  Hepzyme can neutralize up to 2 USP units of unfractionated heparin in 1 mL of citrated plasma.

CPT

85525

CDM Code

80105978

Epic Test ID

1230101316

LOINC

14979-9

Beaker LOINC

APTT NO NEUTRALIZATION: 14979-9

Mayo Access Code

SHO11684

Reviewed Date

10/11/2021

Beaker Names

Beaker Procedure Name: APTT HEPARIN NEUTRALIZATION
Beaker Display Name: APTT Heparin Neutralization
BEAKER TEST NAME: APTT HEPARIN NEUTRALIZATION
BEAKER TEST REPORT NAME: APTT Heparin Neutralization

Beaker Synonyms

No synonym on file

Beaker Location, Container and Temperature

BW COAGULATION: BLUE TOP TUBE (Preferred)-Ambient