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Epic Code LAB3404 MGMT Methylation Analysis

Test Name Alias

O6-methylguanine DNA methyltransferase | | 706

Interface Order Alias

11710

Quick Collect

Tissue/Other – LOOK+LOOK Paraffin Block – Mark Tumor and Normal

Clinical Information

Tissue sections are examed by a Pathologist to scribe relevant tumor.  Pyrosequencing is used to detect methylation status in the promoter region of the O6-methylguanine-DNA-methyltransferase  (MGMT) gene. Aberrant methylation of the CpG islands in the promoter of MGMT gene inactivates the expression of this DNA repair enzyme and sensitizes the tumor to alkylating drugs such as temozolomide. NCCN guideline recommends patients opting for chemotherapy should receive temozolomide if they have MGMT methylation.
 

Specimen Requirements

Specimen Type:  Formalin-fixed, paraffin-embedded (FFPE) tissue (block or 10 unstained slides) accompanied by an H&E slide scribed by Pathologist and Surgical Specimen Testing order sheet.

Specimen Transport Temperature

Ambient

Specimen Stability

Laboratory Retention: Extracted DNA retained greater than 1 year.

Reflex Information

Mandatory Reflex Information:

●   All glioblastoma Grade 4, MGMT Methylation Analysis

Test Frequency

Available once per week (Monday - Friday), usual TAT less than 7 days

Reference Range

An interpretative report will be provided

Performing Department

Molecular Diagnostics

Performing Department Laboratory Location

Corewell Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Pyrosequencing

 

CPT

81287

CDM Code

3008128701

Epic Test ID

1230101493

LOINC

LOINC code not available at this time

Mayo Access Code

SHO11710

Reviewed Date

6/22/2023