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Epic Code LAB343 T-Cell Subsets

Test Name Alias

CD4 and CD8 (CD4, CD3, CD8, CD19) | Helper Suppressor by Flow Cytometry | T Cell Subsets | T and B cells | Lymphocyte Subset Enumeration | Quantitative CD4 and CD8 | CD4 Count | CD8 Count | 4058

Interface Order Alias

11291

Collection Instructions

Specimen Collection: Blood

 

Container(s): Lavender top (EDTA)

Preferred Volume to Collect: 2.0 mL

Minimum Volume to Collect: 1.0 mL

Neonate Volume to Collect: 0.5 mL

Capillary collect ok? No

Microtainer acceptable? Yes

 

Collection Instructions:

  • Gently invert EDTA tube(s) 8 - 10 times after collection.
  • Transport as soon as possible
  • Test must be performed within 48 hours of collection. Testing is not available on Sundays.

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Processed Specimen: Whole Blood EDTA

Centrifuge/Spin: No

Aliquot: No

Transport Temperature: Ambient (transport as soon as possible)

Rejection Criteria

  • Refrigerated
  • Clotted
  • Grossly hemolyzed

Specimen Stability

Ambient: 48 hours

Note: Specimen must remain ambient as all times and testing must be performed within 48 hours of collection

Test Frequency

Monday – Friday 8:00am-4:00pm, usual TAT 2 days.

Reference Range

Normal Lymphocyte Subset Percentages of Peripheral Blood Lymphocytes

  0-3 mos 3-6 mos 6-12 mos 1-2 yrs 2-6 yrs 6-12 yrs 12-18 yrs 18+ yrs
CD3% 53-84 51-77 49-76 53-75 56-75 60-76 56-84 56-86
CD19% 6-32 11-41 14-37 16-35 16-35 13-27 6-23 5-22
CD16/56% 4-18 3-14 3-15 3-15 3-15 4-17 3-22 5-26
CD3/CD4% 35-64 35-56 31-56 32-51 32-51 31-47 31-52 33-58
CD3/CD8% 12-28 12-23 12-24 14-30 14-30 18-35 18-35 13-39
CD4/CD8 ratio 0.9-2.9 0.9-2.9 0.9-2.9 0.9-2.9 0.9-2.9 0.9-2.9 0.9-2.9 0.9-2.9

 

Normal Lymphocyte Subset Absolute Counts of Peripheral Blood Lymphocytes (cells/µL)

 

  0-3 mo 3-6 mo 6-12 mo 1-2 yr 2-6 yr 6-12 yr 12-18 yr 18+ yrs 
CD3 2500-5500 2500-5600 1900-5900 2100-6200 1400-3700 1200-2600 1000-2200 723-2764
CD19 300-2000 430-3000 610-2600 720-2600 390-1400 270-860 110-570 80-616
CD16/56 170-1100 170-830 160-950 180-920 130-720 100-480 70-480 84-724
CD3/CD4 1600-4000 1800-4000 1400-4300 1300-3400 700-2200 650-1500 530-1300 404-1612
CD3/CD8 560-1700 590-1600 500-1700 620-2000 490-1300 370-1100 330-920 220-1129

 

Reference:

Lymphocyte subsets in healthy children from birth through 18 years of age:

The Pediatric AIDS Clinical Trials Group P1009 study

Performing Department

FLOW CYTOMETRY

Performing Department Laboratory Location

Spectrum Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Flow Cytometry

CPT

86360 86355 86359

CDM Code

4028300, 4148366, 4028227

Epic Test ID

1230100459

LOINC

Reporting Name LOINC code
White Cell Count 6690-2
Lymphs from Auto Diff % N/A
Lymphs from Auto Diff Count N/A
CD19 Total B Lymphocytes % 8117-4
CD19 Total B Lymphocytes Cell Count 8116-6
CD3 Mature T Lymphocytes % 8124-0
CD3 Mature T Lymphocytes Cell Count 8122-4
CD4 Helper/Inducer Cells also CD3+ % 8123-2
CD4 Helper/Inducer Cells also CD3+ Count 24467-3
CD8 Suppressor/Cytotoxic also CD3+ % 8101-8
CD8 Suppressor/Cytotoxic also CD3+ Count 14135-8
Helper/Suppressor Ratio N/A

 

Beaker LOINC

CD19+ B CELLS %: 8117-4
CD19+ B CELLS ABSOLUTE COUNT: 8116-6
CD3+ T CELLS %: 8124-0
CD3+ T CELLS ABSOLUTE COUNT: 8124-0
CD4/CD8 RATIO: N/A
CD4+ CD3+ T CELLS %: 8123-2
CD4+ CD3+ T CELLS ABSOLUTE COUNT: 24467-3
CD8+ CD3+ T CELLS %: 8101-8
CD8+ CD3+ T CELLS ABSOLUTE COUNT: 14135-8
LYMPHOCYTE ABSOLUTE COUNT: 731-0
LYMPHOCYTE AUTOMATED: 736-9
WHITE BLOOD CELL: 6690-2

Mayo Access Code

SHO4058

Reviewed Date

7/31/2021

Beaker Names

Beaker Procedure Name: T-CELL SUBSETS BY FLOW CYTOMETRY
Beaker Display Name: T-Cell Subsets by Flow Cytometry
BEAKER TEST NAME: T-CELL SUBSETS BY FLOW CYTOMETRY
BEAKER TEST REPORT NAME: T-Cell Subsets by Flow Cytometry

Beaker Synonyms

CD4|CD8|CD4, CD3, CD8, AND CD19

Beaker Collection and Specimen Handling

BEAKER COLLECTION: +A

Beaker Location, Container and Temperature

LC FLOW CYTOMETRY: 3 ML LAVENDER TOP TUBE A (Preferred)-Ambient