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Epic Code LAB3440 ADAMTS13 Activity

Important Note

There is currently a nationwide shortage of this tube. Please take steps to ensure specimen integrity to reduce recollection. Specimen Tube Shortage (link)

Test Name Alias

Metalloproteinase | AdamTS13 | VWF Cleaving Protease Activity | 7007

Interface Order Alias

11724

Specimen Requirements

Specimen Type: Platelet Poor Plasma
Container/Tube: Blue top (3.2% Sodium Citrate)
Volume to draw: 2.7 mL Whole Blood (Tube must be full) or  1.8 mL Whole Blood (Tube must be full)
Requested Volume: 3 0.5mL aliquots of plasma

Minimum volume: 2 0.4 mL aliquots of plasma

Collection Instructions: Centrifuge and remove plasma within 1 hour of collection. Freeze aliquot in plastic transport tubes immediately

Specimen Transport Temperature

Frozen

Special Instructions

Patient should be off warfarin (Coumadin) for at least 2 weeks.

 

To obtain a coagulation plasma sample, centrifuge the capped specimen tube at a speed and time required to consistently produce platelet-poor plasma (platelet count < 10,000/uL). The centrifugal speed and duration to accomplish this must be established in the individual laboratory. Our laboratories use centrifuges at 3,000 to 4,000 rpm for ten minutes or higher speed and shorter duration centrifuges.  When removing plasma to send for coagulation testing, it is important NOT to go all the way down to the cells. Leave a minimum 1/4 inch of plasma sitting on cells. This layer contains the platelets which were separated from the plasma during spinning. The platelets interfere with coagulation testing so we leave this layer undisturbed.

Test Frequency

Test performed Monday - Friday. Turn-around time 3 - 5 days.

Reference Range

ADAMTS13 Activity ≥ 67%

Performing Department

REFERRAL SEND OUTS

Performing Department Laboratory Location

Send Out to Versiti Wisconsin

Methodology

ADAMTS13 activity is measured by Fluorescence Resonance Energy Transfer (FRET) with a synthetic substrate.7 ADAMTS13 inhibitor is determined using mixing studies; one inhibitor unit is defined as the concentration of inhibitor able to reduce the ADAMTS13 activity of an equal volume of normal pooled plasma by half.8 The ADAMTS13 antibody assay detects IgG antibody serologically by ELISA.

CPT

85397

85335-Factor inhibitor test (if appropriate)

83520-Antibody  (if appropriate)

Epic Test ID

1230100295

Beaker LOINC

Send Out test to Versiti Wisconsin, LOINC Unavailable

Reviewed Date

1/24/2020

Beaker Names

Beaker Procedure Name: ADAMTS13 ACTIVITY
Beaker Display Name: ADAMTS13 Activity
BEAKER TEST NAME: ADAMTS13 ACTIVITY
BEAKER TEST REPORT NAME: ADAMTS13 Activity

Beaker Synonyms

No synonym on file

Beaker Collection and Specimen Handling

BEAKER COLLECTION: LOOK+LOOK

Beaker Location, Container and Temperature

VERSITI WISCONSIN FROZEN: BLUE TOP TUBE (Preferred)