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Test Code LAB3540 Antinuclear Antibodies, HEp-2 Substrate with Reflex

Test Name Alias

ANA | IFA ANA Blood Level | RNAAutoantibodies | ANA Titer: ANA Pattern | 1122 | ANA Hep2 IFA

Collection Instructions

Specimen Collection: Blood

Container(s): Gold Top (Serum Separator-SST Gel)

Preferred Volume to Collect: 5.0 mL

Minimum Volume to Collect: 2.0 mL

Neonate Volume to Collect: 2.0 mL

Capillary collect ok: Yes

Microtainer acceptable: Yes

Collection Instructions:

  • After collection, gently invert tube 8-10 times.
  • Specimen should be processed within 2 hours – See Processing Instructions.

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Processed Specimen: Serum

Centrifuge/Spin: Yes

Aliquot: Yes

Processing Instructions:

  • Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.
  • Aliquot serum into screw top plastic transport tube.

Transport Temperature: Ambient

Rejection Criteria

Specimen not centrifuged within 2 hours

Reflex Information

Reflex approved by Medical Executive Committee. Please see link: Reflex Testing Document

Specimen Stability

Ambient: 8 hours

Refrigerate: 7 days

Frozen: 1 month

Retention: 7 days, positive specimens are frozen for 30 days.

Test Frequency

Performed 3x per week

Turn around time 1 - 4 days

Reference Range

Negative <1:80

Performing Department


Performing Department Laboratory Location

Spectrum Health Regional Laboratory, Grand Rapids, MI


Immunofluorescence Assay (IFA)




86039 if reflex

CDM Code


Epic Test ID


Interface Order Alias


Interface Information

Order: 11778

Result: 21719  - Antinuclear Ab

If reflex

20029 - ANA Pattern

20030 - ANA Titer

Mayo Access Code



Antinuclear Ab: 9423-5

ANA Titer: 5048-4

ANA Pattern: 13068-2

Reviewed Date