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Test Code LAB3540 Antinuclear Antibodies, HEp-2 Substrate with Reflex

Test Name Alias

ANA | IFA ANA Blood Level | RNAAutoantibodies | ANA Titer: ANA Pattern | 1122 | ANA Hep2 IFA

Collection Instructions

Specimen Collection: Blood

Container(s): Gold Top (Serum Separator-SST Gel)

Preferred Volume to Collect: 5.0 mL

Minimum Volume to Collect: 2.0 mL

Neonate Volume to Collect: 2.0 mL

Capillary collect ok: Yes

Microtainer acceptable: Yes

Collection Instructions:

  • After collection, gently invert tube 8-10 times.
  • Specimen should be processed within 2 hours – See Processing Instructions.

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Processed Specimen: Serum

Centrifuge/Spin: Yes

Aliquot: Yes

Processing Instructions:

  • Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.
  • Aliquot serum into screw top plastic transport tube.

Transport Temperature: Ambient

Rejection Criteria

Specimen not centrifuged within 2 hours

Reflex Information

Reflex approved by Medical Executive Committee. Please see link: Reflex Testing Document

Specimen Stability

Ambient: 8 hours

Refrigerate: 7 days

Frozen: 1 month

Retention: 7 days, positive specimens are frozen for 30 days.

Test Frequency

Performed 3x per week

Turn around time 1 - 4 days

Reference Range

Negative <1:80

Performing Department

IMMUNOCHEMISTRY

Performing Department Laboratory Location

Spectrum Health Regional Laboratory, Grand Rapids, MI

Methodology

Immunofluorescence Assay (IFA)

 

CPT

86038

86039 if reflex

CDM Code

4088897

Epic Test ID

1230100353

Interface Order Alias

11778

Interface Information

Order: 11778

Result: 21719  - Antinuclear Ab

If reflex

20029 - ANA Pattern

20030 - ANA Titer

Mayo Access Code

SHO11778

LOINC

Antinuclear Ab: 9423-5

ANA Titer: 5048-4

ANA Pattern: 13068-2

Reviewed Date

9/29/2017