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Test Code LAB3549 Herpes Simplex PCR for Lesions

Test Name Alias

HSV | Herpes Lesions DNA Probe | HSV Lesions DNA Probe | 7025

Icons & Photos

 3-mL UTM/Swab set (UTM medium with Regular Flocked Swab)

#652889

Collection Instructions

Specimen Type: Cutaneous and mucocutaneous lesions from symptomatic patients

 

Container/Tube: 3-mL UTM/Swab set (UTM medium with Regular Flocked Swab)

 

Collection Instructions:

  • Crusted lesions should have the crust removed by lifting with a sterile needle.
  • A swab pre-moistened with sterile saline can be used to collect in areas lacking visible lesions.
  1. Rotate the swab tip several times across the mucosal surface.
  2. Remove the swab and insert into the red top tube of the viral transport medium (UTM) immediately.
  3. Push the swab into the tube and break the plastic shaft swab at the break line.
  4. Replace cap and screw on tightly.
  5. Label specimen with at least 2 patient identifiers (usually name and date of birth). Indicate source.
  6. Store refrigerated. If transport exceeds 24 hours, freeze specimens at -70oC

 

Note:

  • Creams, ointments, lotions, ice, alcohol, Betadine solution, zinc, or a sitz bath reduces viral recovery.
  • Blood introduced into specimen can be inhibitory.
  • Calcium alginate swabs or swabs with wooden shafts should not be used because they may inhibit Herpes viral replication.
  • Viral transport media (UTM) is collection and transport tube of choice. M4 RT is acceptable for OB/GYN clients.

  • CSF is not an acceptable specimen type

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Transport Temperature: Refrigerated or On ice

Specimen Stability

Ambient: Not Acceptable

Refrigerate: 15 days

Frozen: greater than 15 days

Laboratory Retention: 3 days

Test Frequency

Available daily, usual TAT 1 day.

Reference Range

Negative for HSV-1 and HSV-2

 

Critical Values Table

Performing Department

MICROBIOLOGY

Performing Department Laboratory Location

Spectrum Health Regional Laboratory, Grand Rapids, MI

Methodology

DNA Amplification

CPT

87529

CDM Code

4118877

Epic Test ID

1230100809

Interface Order Alias

55010

LOINC

HSV-1 DNA          16130-7

HSV-2 DNA          16131-5

Mayo Access Code

SHO55010

Additional Information

Also results for HSV1 and HSV2

Reviewed Date

3/18/2020