Sign in →

Test Code LAB3584 Barbiturates Urine Level

Test Name Alias

LCMS Barbiturates | Butalbital | Secobarbital | Pentobarbital | Phenobarbital | Confirmation | 503

Collection Instructions

Specimen Collection: Urine


Container(s):  Sterile Urine Cup / Plastic screw-top transport tube

Preferred Volume to Collect: 10.0 mL

Minimum Volume to Collect: 1.0 mL


Collection Instructions:

  • Urine specimen must be collected on site at lab or office. Do not send collection container home with patient.
  • Keep container upright at all times and check that lid is fastened evenly and securely.

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Transport Temperature: Refrigerate

Specimen Stability

Ambient: 72 hours

Refrigerate: 14 days

Frozen: 365 days

Laboratory Retention: 10 days

Test Frequency

Available Monday through Friday, day shift. TAT 2 – 3 days

Reference Range

Barbiturates tested for are Butalbital, Secobarbital, Pentobarbital and Phenobarbital.


A urine barbiturate (Butalbital/Secobarbital/Pentobarbital/Phenobarbital) result of < 300 is a NEGATIVE result (300 ng/mL is the lower reporting limit for this LCMS confirmation assay).  


Values reported ≥ to 300 should be interpreted as POSITIVE.

Performing Department


Performing Department Laboratory Location

Spectrum Health Regional Laboratory, Grand Rapids, MI


Liquid Chromatography Mas Spectrometry (LC-MS)


This test was developed and its performance characteristics determined by Spectrum Health Toxicology Laboratory. It has not been cleared or approved by the FDA. The laboratory is regulated under CLIA as qualified to perform high-complexity testing. This test is used for clinical purposes. It should not be regarded as investigational or for research.


Linked chargeables: HC BARBITURATES CONFIRMATION [80345 (CPT®)]

CDM Code


Epic Test ID


Interface Order Alias



Barbiturates Conf: 16429-3

Mayo Access Code


Reviewed Date