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Epic Code LAB3611 MPN Expanded Panel

Important Note

This test does not include testing for JAK2 V617F.  If JAK2 V617F testing has not been performed previously, order JAK2 V617F Mutation, do MPN if Negative (Test code 7135).

The same sample may be used for both JAK2 V617F and MPN Expanded Panel testing.

Test Name Alias

MPN Expanded Panel | JAK2 V617F Quantitative PCR, do MPN if Negative | CALR MPN Expanded Panel | JAK2 Exon 12 MPN Expanded Panel | 7090 | MPL MPN Expanded Panel

Interface Order Alias

55043

Clinical Information

The MPN Expanded panel assay uses next generation sequencing and fragmentation analysis to detect well defined mutations in CALR exon 9, JAK2 exon 12 and MPL exon 10, which, in addition to BCR-ABL gene fusion and JAK2 V617F mutation testing, aids in the diagnosis of Myeloproliferative Neoplasms.

Specimen Requirements

Submit only one of the following specimens: (Blood or Bone Marrow)

Specimen Collection: Blood

Container(s): Lavender Top (EDTA)

Preferred Volume to Collect: 6.0 mL

Minimum Volume to Collect: 4.0 mL

Collection Instructions:

  • Gently invert EDTA tube(s) 8 - 10 times after collection.
  • For optimal results, the specimens should be received in the laboratory within 48 hours of collection

Processing Instructions (Laboratory, Outpatient or Off-site collection)

  • Processed Specimen: Whole Blood EDTA
  • Centrifuge/Spin: No
  • Aliquot: No
  • Transport Temperature: Ambient or Refrigerated

 

Specimen Collection: Bone Marrow

Container(s): Lavender Top (EDTA)

Preferred Volume to Collect: 6.0 mL

Minimum Volume to Collect: 4.0 mL

Collection Instructions:

  • For optimal results, the specimens should be received in the laboratory within 48 hours of collection

Processing Instructions (Laboratory, Outpatient or Off-site collection)

  • Transport Temperature: Ambient or Refrigerated

Specimen Stability

Ambient: 24 hours

Refrigerate: 72 hours

Laboratory Retention: Extracted DNA retained greater than 1 year

Reflex Information

Link: MEC Approved Reflex Testing 

Test Frequency

Available once per week, usual TAT less than 7 days

Reference Range

An interpretative report will be provided.

Performing Department

MOLECULAR DIAGNOSTICS

Performing Department Laboratory Location

Spectrum Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Next Generation Sequencing by Ion AmpliSeq technology and fragmentation analysis

CPT

HC CALR VAR IN EXON 9 [81219 (CPT®)]
HC JAK2 GENE TRGT SEQUENCE ALYS [81279 (CPT®)]
HC MPL GENE COMMON VARIANTS [81338 (CPT®)]

CDM Code

 80101195 - HC CALR VAR IN EXON 9 

80106151 - HC JAK2 GENE TRGT SEQUENCE ALYS

80106152 - HC MPL GENE COMMON VARIANTS

Epic Test ID

1230100954

LOINC

Not available

Beaker LOINC

CALR: 77174-1
JAK2 EXON 12: 63421-2
MPL: 75033-1

Mayo Access Code

SHO55043

Reviewed Date

8/23/2021

Beaker Names

Beaker Procedure Name: MPN EXPANDED PANEL
Beaker Display Name: MPN Expanded Panel
BEAKER TEST NAME: MPN EXPANDED PANEL
BEAKER TEST REPORT NAME: MPN Expanded Panel

Beaker Synonyms

No synonym on file

Beaker Collection and Specimen Handling

BEAKER COLLECTION: Blood or Bone Marrow = +A Other = Call lab for instruction

Beaker Location, Container and Temperature

LC MOLECULAR DIAGNOSTICS: 3 ML LAVENDER TOP TUBE A (Preferred)-Ambient
LC MOLECULAR DIAGNOSTICS: FORMALIN 10% (Acceptable)-Ambient
LC MOLECULAR DIAGNOSTICS: STERILE CONTAINER A (Acceptable)-No Temperature