Epic Code LAB3618 Enteric Pathogens by PCR
Test Name Alias
Enteric Pathogens by PCR | Stool Culture | 7122
Interface Order Alias
55078
Clinical Information
The qualitative nucleic acid test (utilizing reverse transcriptase PCR) detects genetic markers of common community-acquired pathogenic enteric bacteria, viruses from a stool sample, two to three days faster than current methods:
Icons & Photos
Cary Blair Vial of the Stool Specimen Kit. (Contains Cary Blair Medium, pink liquid)
Always READ LABELS as container colors may differ based on manufacturer.
Collection Instructions
Specimen Type: Preserved Stool
Container/Tube: Cary-Blair Medium
Volume: Fill container to volume indicated on vial
Collection Instructions:
- Collect liquid or soft stool preserved in Cary-Blair medium by using the manufacturer's recommended collection procedure to fill to the line indicated halfway up on the container.
- Note: This panel may not be utilized as a test of cure and repeat testing will not be performed on samples collected <7 days apart.
- Note: For small sample volume, remove some of the media and then add stool, trying to maintain the 1 to 3 ratio of stool to media. Make an order note in the Laboratory Information System that media was poured out before the sample was added.
Specimen Transport Temperature: Ambient
Lab Staff Instructions
- Cary-Blair preserved specimens to be stored refrigerated at 2 - 8°C until testing is completed (for up to 48 hours after collection)
Specimen Stability
Ambient: 48 hours
Refrigerate: 48 hours
Frozen: do not freeze
Laboratory Retention: 3 days
Test Frequency
The test is performed first and second shift at SHRL. Turn around time: 24 hours upon receipt in laboratory
Reference Range
Detects genetic markers of the following:
• Campylobacter Group (composed of C. coli, C. jejuni, and C. lari)
• Salmonella species
• Shigella species (including S. dysenteriae, S. boydii, S. sonnei, and S. flexneri)
• Vibrio Group (composed of V. cholerae and V. parahaemolyticus)
• Yersinia enterocolitica
• Norovirus GI/GII
• Rotavirus A
In addition, EP detects the Shiga toxin 1 gene and Shiga toxin 2 gene virulence markers. Shiga toxin producing E. coli (STEC) typically harbor one or both genes that encode for Shiga toxins 1 and 2.
If Aeromonas is a concern, call the Microbiology laboratory ahead of tme at 616-267-2640 and order Microbiology organism identification reference [LAB4003] in conjuction with ordering the Enteric pathogen panel.
Performing Department
MICROBIOLOGY
Performing Department Laboratory Location
Spectrum Health Regional Laboratory, Grand Rapids, MI
Methodology
The qualitative nucleic acid test (utilizing reverse transcriptase PCR) to detect genetic markers.
CPT
87506
CDM Code
4048879
Epic Test ID
123001714
LOINC
Not available
Beaker LOINC
CAMPYLOBACTER GROUP: 79382-8
NOROVIRUS GI/GII: 79388-5
ROTAVIRUS: 72174-6
SALMONELLA SPECIES: 79383-6
SHIGA TOXIN 1: 79386-9
SHIGA TOXIN 2: 79387-7
SHIGELLA SPECIES: 70242-3
VIBRIO GROUP: 79384-4
YERSINIA ENTEROCOLITICA: 79385-1
Mayo Access Code
SHO55078
Additional Information
- Policy Tech: 20065
- Enteric Pathogen Panel replaces stool culture
Reviewed Date
7/31/2021
Beaker Names
Beaker Procedure Name: ENTERIC PATHOGENS BY PCR
Beaker Display Name: Enteric Pathogens by PCR
BEAKER TEST NAME: ENTERIC PATHOGENS PCR
BEAKER TEST REPORT NAME: Enteric Pathogens PCR
Beaker Synonyms
No synonym on file
Beaker Location, Container and Temperature
BW MICROBIOLOGY: CARY-BLAIR MEDIA (Preferred)-Ambient