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Epic Code LAB490 Protein C Antigen

Important Note

There is currently a nationwide shortage of this tube. Please take steps to ensure specimen integrity to reduce recollection. Specimen Tube Shortage (link)

Patient should be off warfarin (Coumadin) for at least 2 weeks.

Versiti Wisconsin Order Code: 1033

Test Name Alias

Protein C Antigen Level | 4174

Interface Order Alias

10459

Clinical Information

Differentiation of congenital from acquired deficiency requires clinical corelation. Causes of acquired protein C deficiency include acute thrombosis, DIC, liver disease, vitamin k deficiency, and warfarin anticoagulation. Protein C antigen is not a functional assay.

Specimen Requirements

Specimen Type: Platelet Poor Plasma
Container/Tube: Blue top (3.2% Sodium Citrate)
Volume to collect: 2.7 mL Whole Blood (Tube must be full) or  1.8 mL Whole Blood (Tube must be full)
Prefered volume: 0.5mL plasma

Minimum volume: 0.3mL plasma

Collection Instructions: Centrifuge and remove plasma within 1 hour of collection. Freeze aliquot in plastic transport tubes immediately

COLLECTION NOTE: Volumes listed are in serum or plasma, draw approximately 2 1/2 times the requested volume in whole blood.

Special Instructions

Patient should be off warfarin (Coumadin) for at least 2 weeks.

 

To obtain a coagulation plasma sample, centrifuge the capped specimen tube at a speed and time required to consistently produce platelet-poor plasma (platelet count < 10,000/uL). The centrifugal speed and duration to accomplish this must be established in the individual laboratory. Our laboratories use centrifuges at 3,000 to 4,000 rpm for ten minutes or higher speed and shorter duration centrifuges.  When removing plasma to send to for coagulation testing, it is important NOT to go all the way down to the cells. Leave a minimum 1/4 inch of plasma sitting on cells. This layer contains the platelets which were separated from the plasma during spinning. The platelets interfere with coagulation testing so we leave this layer undisturbed.

Rejection Criteria

Hemolysis

Specimen Stability

Ambient: 1 hour

Frozen: 2 months

Test Frequency

Sent to reference laboratory. Usual TAT 7-10 days.

Reference Range

≥ 73 u/dL

Performing Department

REFERRAL SEND OUTS

Performing Department Laboratory Location

Send Out to Versiti Wisconsin Ref. Lab

Methodology

ELISA

CPT

85302

CDM Code

80101918

Epic Test ID

1230101082

LOINC

Protein C Antigen: 6009-5

Beaker LOINC

Send Out test to Versiti Wisconsin , LOINC Unavailable

Reviewed Date

1/24/2020

Beaker Names

Beaker Procedure Name: PROTEIN C ANTIGEN
Beaker Display Name: Protein C Antigen
BEAKER TEST NAME: PROTEIN C ANTIGEN
BEAKER TEST REPORT NAME: Protein C Antigen

Beaker Synonyms

No synonym on file

Beaker Collection and Specimen Handling

BEAKER COLLECTION: LOOK+LOOK

Beaker Location, Container and Temperature

VERSITI WISCONSIN FROZEN: BLUE TOP TUBE