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Test Code LAB535 Vitamin D 25 Hydroxy Level (25-OH Vitamin D)

Test Name Alias

Vitamin D 25-Hydroxy Blood Level | VitaminD25Lvl | VitaminD25 Blood Level | Vitamin D | VitaminD | 8330 | Calcifediol

Clinical Information

Vitamin D (25-Hydroxy) is the preferred test for the assessment of Vitamin D absorption and body stores.

Specimen Requirements

Specimen Type: Serum
Container/Tube: Gold Top (Serum Separator-SST Gel)
Volume: 5 mL
Minimum volume: 1.0 mL serum
Collection Instructions: Allow specimen to clot for 30 minutes and centrifuge within 2 hours

Specimen Transport Temperature


Specimen Stability

Ambient: 8 hours

Refrigerate: 72 hours

Frozen: aliquot serum for longer storage

Laboratory Retention: 72 hours

Test Frequency

Available 24 hours, usual TAT 1 day.

Reference Range

  • <10 ng/mL Vitamin D:  Severe Deficiency, risk for osteomalacia or rickets
  • 10 – 19 ng/mL Vitamin D: Moderate to Marginal Deficiency, risk for osteoporosis or secondary hyperparathyroidism
  • 20 – 50 ng/mL Vitamin D: Sufficient level
  • 50 – 80 ng/mL Vitamin D: Possible hypercalciuria, risk with prolonged calcium supplementation
  • >80 ng/mL Vitamin D: Toxicity possible, lowest level with reported toxicity

Population based distributions of total 25-OH Vitamin D levels in the Grand Rapids area, based on studies performed in the winter (February) and summer (August), are 8 to 32 ng/mL and 8 to 48 ng/mL respectively. Vitamin D levels are subject to a significant seasonable variation due to differences in sun exposure.

Performing Department


Performing Department Laboratory Location

Spectrum Health Regional Laboratory, Grand Rapids, MI
Spectrum Health Big Rapids Laboratory, Big Rapids, MI
Spectrum Health Gerber Laboratory, Fremont, MI
Spectrum Health Ludington Laboratory, Ludington, MI
Spectrum Health Pennock Laboratory, Hastings, MI
Spectrum Health Reed City Laboratory, Reed City, MI
Spectrum Health United Laboratory, Greenville, MI
Spectrum Health Zeeland Laboratory, Zeeland, MI


Electrochemiluminescence Immunoassay (ECLIA)



CDM Code


Epic Test ID


Interface Order Alias



25-OH Vitamin D Total: 62292-8

Mayo Access Code


Reviewed Date