Test Code LAB535 Vitamin D 25 Hydroxy Level (25-OH Vitamin D)
Test Name Alias
Vitamin D 25-Hydroxy Blood Level | VitaminD25Lvl | VitaminD25 Blood Level | Vitamin D | VitaminD | 8330 | Calcifediol
Clinical Information
Vitamin D (25-Hydroxy) is the preferred test for the assessment of Vitamin D absorption and body stores.
Specimen Requirements
Specimen Type: Serum
Container/Tube: Gold Top (Serum
Separator-SST Gel)
Volume: 5 mL
Minimum volume: 1.0 mL serum
Collection Instructions: Allow
specimen to clot for 30 minutes and centrifuge within 2 hours
Specimen Transport Temperature
Refrigerate
Specimen Stability
Ambient: 8 hours
Refrigerate: 72 hours
Frozen: aliquot serum for longer storage
Laboratory Retention: 72 hours
Test Frequency
Available 24 hours, usual TAT 1 day.
Reference Range
- <10 ng/mL Vitamin D: Severe Deficiency, risk for osteomalacia or rickets
- 10 – 19 ng/mL Vitamin D: Moderate to Marginal Deficiency, risk for osteoporosis or secondary hyperparathyroidism
- 20 – 50 ng/mL Vitamin D: Sufficient level
- 50 – 80 ng/mL Vitamin D: Possible hypercalciuria, risk with prolonged calcium supplementation
- >80 ng/mL Vitamin D: Toxicity possible, lowest level with reported toxicity
Population based distributions of total 25-OH Vitamin D levels in the Grand Rapids area, based on studies performed in the winter (February) and summer (August), are 8 to 32 ng/mL and 8 to 48 ng/mL respectively. Vitamin D levels are subject to a significant seasonable variation due to differences in sun exposure.
Performing Department
CHEMISTRY
Performing Department Laboratory Location
Spectrum Health Regional Laboratory, Grand Rapids, MI
Spectrum Health Big Rapids Laboratory, Big Rapids, MI
Spectrum Health Gerber Laboratory, Fremont, MI
Spectrum Health Ludington Laboratory, Ludington, MI
Spectrum Health Pennock Laboratory, Hastings, MI
Spectrum Health Reed City Laboratory, Reed City, MI
Spectrum Health United Laboratory, Greenville, MI
Spectrum Health Zeeland Laboratory, Zeeland, MI
Methodology
Electrochemiluminescence Immunoassay (ECLIA)
CPT
82306
CDM Code
4058838
Epic Test ID
1230101291
Interface Order Alias
10807
LOINC
25-OH Vitamin D Total: 62292-8
Mayo Access Code
SHO8330
Reviewed Date
3/5/2020