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Epic Code LAB535 Vitamin D 25 Hydroxy Level (25-OH Vitamin D)

Test Name Alias

Vitamin D 25-Hydroxy Blood Level | VitaminD25Lvl | VitaminD25 Blood Level | Vitamin D | VitaminD | 8330 | Calcifediol

Interface Order Alias


Clinical Information

Vitamin D (25-Hydroxy) is the preferred test for the assessment of Vitamin D absorption and body stores.

Collection Instructions

Specimen Collection: Blood


Container(s): 5.0 mL Gold Top (Serum Separator-SST Gel)

  • Preferred Volume to Collect: 5.0 mL
  • Minimum Volume to Collect: 2.0 mL
  • Neonate (NICU) Volume to Collect: at least 1 full microtainer (not shared)
  • Capillary collect ok? Yes
  • Microtainer acceptable: Yes


Collection Instructions:

  • Gently invert 8 - 10 times to mix clot activator with blood.
  • Specimen should be processed within 2 hours – See Processing Instructions.


Processing Instructions (Laboratory, Outpatient or Off-site collection)

  • Processed Specimen: Serum
  • Centrifuge/Spin: Yes
  • Aliquot: Yes
  • Processing Instructions:
    • Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.
    • Minimum serum volume: 1.0 mL serum
  • Transport Temperature: Refrigerate

Specimen Stability

Ambient: 8 hours

Refrigerate: 72 hours

Frozen: aliquot serum for longer storage

Laboratory Retention: 72 hours

Test Frequency

Available 24 hours, usual TAT 1 day.

Reference Range

  • <10 ng/mL Vitamin D:  Severe Deficiency, risk for osteomalacia or rickets
  • 10 – 19 ng/mL Vitamin D: Moderate to Marginal Deficiency, risk for osteoporosis or secondary hyperparathyroidism
  • 20 – 50 ng/mL Vitamin D: Sufficient level
  • 50 – 80 ng/mL Vitamin D: Possible hypercalciuria, risk with prolonged calcium supplementation
  • >80 ng/mL Vitamin D: Toxicity possible, lowest level with reported toxicity

Population based distributions of total 25-OH Vitamin D levels in the Grand Rapids area, based on studies performed in the winter (February) and summer (August), are 8 to 32 ng/mL and 8 to 48 ng/mL respectively. Vitamin D levels are subject to a significant seasonable variation due to differences in sun exposure.

Performing Department


Performing Department Laboratory Location

Corewell Health Reference Laboratory, Grand Rapids, MI


Electrochemiluminescence Immunoassay (ECLIA)



CDM Code


Epic Test ID



25-OH Vitamin D Total: 62292-8

Mayo Access Code


Reviewed Date