Epic Code LAB535 Vitamin D 25 Hydroxy Level (25-OH Vitamin D)
Test Name Alias
Vitamin D 25-Hydroxy Blood Level | VitaminD25Lvl | VitaminD25 Blood Level | Vitamin D | VitaminD | 8330 | Calcifediol
Interface Order Alias
10807
Clinical Information
Vitamin D (25-Hydroxy) is the preferred test for the assessment of Vitamin D absorption and body stores.
Collection Instructions
Specimen Collection: Blood
Container(s): 5.0 mL Gold Top (Serum Separator-SST Gel)
- Preferred Volume to Collect: 5.0 mL
- Minimum Volume to Collect: 2.0 mL
- Neonate (NICU) Volume to Collect: at least 1 full microtainer (not shared)
- Capillary collect ok? Yes
- Microtainer acceptable: Yes
Collection Instructions:
- Gently invert 8 - 10 times to mix clot activator with blood.
- Specimen should be processed within 2 hours – See Processing Instructions.
Processing Instructions (Laboratory, Outpatient or Off-site collection)
- Processed Specimen: Serum
- Centrifuge/Spin: Yes
- Aliquot: Yes
- Processing Instructions:
- Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.
- Minimum serum volume: 1.0 mL serum
- Transport Temperature: Refrigerate
Specimen Stability
Ambient: 8 hours
Refrigerate: 72 hours
Frozen: aliquot serum for longer storage
Laboratory Retention: 72 hours
Test Frequency
Available 24 hours, usual TAT 1 day.
Reference Range
- <10 ng/mL Vitamin D: Severe Deficiency, risk for osteomalacia or rickets
- 10 – 19 ng/mL Vitamin D: Moderate to Marginal Deficiency, risk for osteoporosis or secondary hyperparathyroidism
- 20 – 50 ng/mL Vitamin D: Sufficient level
- 50 – 80 ng/mL Vitamin D: Possible hypercalciuria, risk with prolonged calcium supplementation
- >80 ng/mL Vitamin D: Toxicity possible, lowest level with reported toxicity
Population based distributions of total 25-OH Vitamin D levels in the Grand Rapids area, based on studies performed in the winter (February) and summer (August), are 8 to 32 ng/mL and 8 to 48 ng/mL respectively. Vitamin D levels are subject to a significant seasonable variation due to differences in sun exposure.
Performing Department
CHEMISTRY
Performing Department Laboratory Location
Corewell Health Reference Laboratory, Grand Rapids, MI
Methodology
Electrochemiluminescence Immunoassay (ECLIA)
CPT
82306
CDM Code
3018230601
Epic Test ID
1230101291
LOINC
25-OH Vitamin D Total: 62292-8
Mayo Access Code
SHO8330
Reviewed Date
3/23/2022