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Test Code LAB782 Fructosamine, Serum

Additional Codes

Mayo Code: FRUCT

Epic Code: LAB 782

Interface Order Alias: 10143

Cerner code: 8612

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Assessing intermediate-term glycemic control

Reporting Name

Fructosamine, S

Specimen Type

Serum


Specimen Required


Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 1 mL

Collection Instructions:

1. Serum gel tubes should be centrifuged within 2 hours of collection.

2. Red-top tubes should be centrifuged and aliquoted within 2 hours of collection.


Specimen Minimum Volume

0.25 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 7 days
  Frozen  7 days
  Ambient  72 hours

Reject Due To

Hemolysis

Mild reject; Gross reject

Lipemia

NA

Icterus

Mild reject; Gross reject

Other

NA

 

Clinical Information

Fructosamine is a general term, which applies to any glycated protein. It is formed by the nonenzymatic reaction of glucose with the a- and e-amino groups of proteins to form intermediate compounds called aldimines. These aldimines may dissociate or undergo an Amadori rearrangement to form stable ketoamines called fructosamines. This nonenzymatic glycation of specific proteins in vivo is proportional to the prevailing glucose concentration during the lifetime of the protein. Therefore, glycated protein measurement in the diabetic patient is felt to be a better monitor of long-term glycemic control than individual or sporadic glucose determinations. The best known of these proteins is glycated hemoglobin which is often measured as hemoglobin A1c, and reflects glycemic control over the past 6 to 8 weeks. In recognition of the need for a measurement that reflects intermediate-term glycemic control and was easily automated, a nonspecific test, termed fructosamine, was developed. Since albumin is the most abundant serum protein, it accounts for 80% of the glycated serum proteins, and thus, a high proportion of the fructosamine. Although a large portion of the color generated in the reaction is contributed by glycated albumin, the method will measure all proteins, each with a different half-life and different levels of glycation.

Reference Values

200-285 mcmol/L

Interpretation

In general, fructosamine reflects glycemic control in diabetic patients over the previous 2 to 3 weeks. High values indicate poor control.

Clinical Reference

1. Tietz Textbook of Clinical Chemistry, Edited by CA Burtis, ER Ashwood. Philadelphia, WB Saunders Company, 1999

2. Austin GE, Mullins RH, Morin LG: Non-enzymatic glycation of individual plasma proteins in normoglycemic and hyperglycemic patients. Clin Chem 1987;33:2220-2224

3. Schleicher ED, Mayer R, Wagner EM, Gerbitz KD: Is serum fructosamine assay specific for determination of glycated serum protein? Clin Chem 1988;34:320-323

Method Description

The fructosamine assay is a colorimetric test based on the ability of ketoamines to reduce nitroblue tetrazolium to formazan in an alkaline medium. The rate of formation of formazan is directly proportional to the concentration of fructosamine, and is measured photometrically at 546 nm.(Package insert: Roche Fructosamine reagent, Roche Diagnostics Corp., Indianapolis, IN 1999)

Method Name

Colorimetric Rate Reaction

Day(s) and Time(s) Performed

Monday through Sunday; Continuously

Analytic Time

Same day/1 day

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

LOINC Code Information

Test ID Test Order Name Order LOINC Value
FRUCT Fructosamine, S 15069-8

 

Result ID Test Result Name Result LOINC Value
FRUCT Fructosamine, S 15069-8

CPT Code Information

82985