Sign in →

Epic Code LAB7850 Bartonella Antibody Panel, IgG and IgM, Serum

Additional Codes

Mayo code: BART

Reporting Name

Bartonella Ab Panel, IgG and IgM

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Clinical Information

Bartonella henselae and Bartonella quintana are small, rod-shaped, pleomorphic, Gram-negative bacteria. The human body louse (Pediculus humanis) is the proposed vector for B quintana. No animal reservoir has been determined for B quintana. The domestic cat is believed to be both a reservoir and vector for B henselae. Cats may infect humans directly through scratches, bites, or licks; or indirectly through an arthropod vector. Humans remain the only host in which Bartonella infection leads to significant disease.


The sight of entry for Bartonella is through openings in the skin. Microscopically, Bartonella lesions appear as rounded aggregates that proliferate rapidly. These aggregates are masses of Bartonella bacteria. Warthin-Starry staining has shown that Bartonella organisms can be present within the vacuoles of endothelial cells, in macrophages, and between cells in areas of necrosis. Occasionally organisms are seen in the lumens of vessels. While cutaneous lesions are common, disseminated tissue infection by Bartonella has been seen in the blood, lymph nodes, spleen, liver, bone marrow, and heart. B henselae has been associated with cat scratch disease (CSD), peliosis hepatitis (PH), bacillary angiomatosis (BA), and endocarditis. B quintana has been associated with trench fever, BA, and endocarditis. BA is a vascular proliferative disease usually involving the skin and regional lymph nodes.


CSD begins as a cutaneous papule or pustule that usually develops within a week after an animal contact. Regional lymphadenopathy follows and is the predominant clinical feature of CSD. Trench fever, which was a significant problem during World War I and World War II, is characterized by a relapsing fever and severe pain in the shins. PH and febrile bacteremia syndrome are both syndromes that have afflicted patients with AIDS and patients who are immunocompromised. While trench fever and CSD are usually self-limiting illnesses, the other Bartonella-associated diseases can be life-threatening.


Interest in B quintana and B henselae has recently increased since its increased prevalence in patients with AIDS, in transplant patients, and those with suppressed immunity.

Useful For

Diagnosis of Bartonella infection, especially in the context of a cat scratch


This assay can be used as an aid to diagnose recent or past infection with Bartonella henselae or Bartonella quintana.

Specimen Type


Specimen Required


Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL

Laboratory Test Directory Note:

COLLECTION NOTE: Volumes listed are in serum or plasma, draw approximately 2 1/2 times the requested volume in whole blood.


LAB: +S/PO/R=Spin at 3500 for 5 minutes after 30 minute rest. Pour into white pour-over tube. Transport and store refrigerated.

Specimen Minimum Volume

0.15 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 30 days
  Frozen  30 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Day(s) Performed

Monday through Saturday

Reference Values

Bartonella henselae

IgG: <1:128

IgM: <1:20


Bartonella quintana

IgG: <1:128

IgM: <1:20


A positive immunofluorescence assay (IFA) IgM (titer >1:20) suggests a current infection with either Bartonella henselae or Bartonella quintana.


A positive IgG (titer >1:128) suggests a current or previous infection. Increases in IgG titers in serial specimens suggest active infection.


Normal serum specimens usually have an IgG titer of less than 1:128. However, 5% to 10% of healthy controls exhibit a B henselae and B quintana titer of 1:128. Sera from healthy volunteers rarely show titers of 1:256 or greater. IgM titers in normal serum are typically less than 1:20. IgM titers at 1:20 or greater have not been seen in the normal population.


Molecular testing of tissue for Bartonella species nucleic acid is recommended in cases of suspected endocarditis.

Clinical Reference

1. Rodino KG, Stone E, Saleh OA, Theel ES: The Brief Case: Bartonella henselae endocarditis-a case of delayed diagnosis. J Clin Microbiol. 2019 Aug 26;57(9). e00114-19. doi: 10.1128/JCM.00114-19

2. Wolf LA, Cherry NA, Maggi RG, Breitschwerdt EB: In pursuit of a stealth pathogen: Laboratory diagnosis of bartonellosis. Clin Micro News. 2014;36(5):33-39

Method Name

Immunofluorescence Assay (IFA)

Method Description

The Euroimmun indirect immunofluorescence test is a standardized in vitro assay for the determination of specific antibodies against Bartonella henselae and Bartonella quintana. BIOCHIP Mosaics are coated with B henselae and B quintana infected cells positioned next to each other in one reaction field. Samples are diluted and incubated on the substrate slides. If the reaction is positive, specific antibodies of class IgG and IgM attach to the antigens. In a second step, the attached antibodies are stained with fluorescein-labeled anti-human antibodies and made visible using fluorescence microscopy. Semi quantitative endpoint titers are obtained by testing serial dilutions of positive specimens.(Package insert: IIFT Bartonella henselae/Bartonella quintana (IgM) Instructions for the indirect immunofluorescence test. Euroimmun US, Inc; Version 7/18/2017)

Report Available

Same day/1 to 3 days

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

86611 x 4

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BART Bartonella Ab Panel, IgG and IgM 90251-0


Result ID Test Result Name Result LOINC Value
15659 Bart Henselae IgG 6954-2
15660 Bart Henselae IgM 6955-9
15661 Bart Quintana IgG 44827-4
15662 Bart Quintana IgM 44825-8


If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Laboratory Test Directory Additional Information: