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Epic Code LAB829 Mycoplasmoides pneumoniae, Molecular Detection, PCR, Varies

Additional Codes

Mayo Code: MPRP

Epic Code: LAB 829

Interface Order Alias: 10575

Cerner code: 4188

 

Spectrum Health Cerner Test Name: Mycoplasma by DNA PCR Sputum (BAL)

Reporting Name

Mycoplasma pneumoniae PCR

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Clinical Information

Mycoplasmoides pneumoniae, previously Mycoplasma pneumoniae, is a small bacterium transmitted via organism-containing droplets. It is a cause of upper respiratory infection, pharyngitis, and tracheobronchitis, particularly in children, and has been associated with approximately 20% of cases of community acquired pneumonia.(1) Central nervous system and cardiac manifestations are probably the most frequent extrapulmonary complications of infections due to M pneumoniae. The disease is usually self-limited although severe disease has been reported in immunocompromised patients.(2)

 

Identification of M pneumoniae by culture-based methods is time consuming and insensitive. Serology based assays for M pneumoniae have several drawbacks. The development of IgM antibodies takes approximately 1 week and the IgM response in adults may be variable or it may be decreased in immunosuppressed individuals.(3,4) Confirmation of the disease may be dependent on the observation of a 4-fold rise in IgG antibody titers between acute and convalescent specimens, several weeks following the initial onset of illness, providing clinical utility only for retrospective testing.(4) Real-time polymerase chain reaction testing offers a rapid and sensitive option for detection of M pneumoniae DNA from clinical specimens.

Useful For

Diagnosis of infections due to Mycoplasmoides pneumoniae

Specimen Type

Varies


Shipping Instructions


 



Necessary Information


Specimen source is required.



Specimen Required


The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Mycoplasmoides pneumoniae DNA is unlikely.

 

Submit only 1 of the following specimens:

 

Specimen Type: Respiratory

Supplies:

-Bartels FlexTrans VTM-3 mL (T892)

-Jiangsu VTM-3 mL (T891)

-M4-RT (T605)

Sources: Bronchial washing, bronchoalveolar lavage, tracheal secretions, sputum

Container/Tube:

Preferred: Sterile container

Acceptable: Specimen in M4, M4-RT, M5, M6, or universal transport medium

Specimen Volume: 1 mL

 

Specimen Type: Swab

Supplies:

-Culturette (BBL Culture Swab) (T092)

-BD Eswab (T853)

-Steriflock NP Swab (T861)

-Nasopharyngeal Swab (Rayon Mini-Tip Swab) (T515)

-Bartels FlexTrans VTM-3 mL (T892)

-Jiangsu VTM-3 mL (T891)

-M4-RT (T605)

Sources: Throat, nasal, or nasopharyngeal

Container/Tube:

Preferred: Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)

Acceptable: Culture transport swab (Stuart's media) or place swab in M4, M4-RT, M5, M6, universal transport media, or ESwab

Specimen Volume: Swab

Collection Instructions:

1. Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.

2. Place swab back into swab cylinder.

 

Specimen Type: Fluid

Sources: Pleural, pericardial, cerebrospinal

Container/Tube: Sterile vial

Specimen Volume: 0.5 mL


Specimen Minimum Volume

Respiratory, Fluid: 0.5 mL
Swab: 1 swab

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

Cotton or calcium alginate-tipped swab, wooden shaft swab, transport swab containing gel or charcoal
Port-a-Cul tube
Anaerobic fluid vials
Dry swab (no pledget or sponge)		 Reject

Day(s) Performed

Monday through Sunday

Reference Values

Not applicable

Interpretation

A positive result indicates the presence of Mycoplasmoides pneumoniae.

 

A negative result does not rule out the presence of M pneumoniae and may be due to the presence of inhibitors within the specimen matrix, or the presence of organisms at numbers below the limits of detection of the assay.

Cautions

This assay should only be used for testing of respiratory tract specimens (throat swabs, nasopharyngeal swabs, tracheal secretions, sputum, and bronchoalveolar lavage fluid) and pleural/chest fluid, pericardial fluid, and cerebrospinal fluid.

Method Name

Rapid Polymerase Chain Reaction (PCR) using Light Cycler and Fluorescent Resonance Energy Transfer (FRET)

Method Description

Throat swabs, nasopharyngeal swabs, sputum, bronchoalveolar lavage fluid, pericardial/pleural/chest fluid, and cerebrospinal fluid specimens are processed according to specimen type. Nucleic acid is extracted by the MagNA Pure automated instrument (Roche Applied Science). A specific target sequence from Mycoplasma pneumoniae is targeted by primers and fluorescence resonance energy transfer (FRET) hybridization probes. The LightCycler instrument (Roche Applied Science) amplifies and monitors the development of target nucleic acid sequences after the annealing step during polymerase chain reaction cycling. Detection of the M pneumoniae target is performed through melting curve analysis using the LightCycler software.(Schmitt BH, Sloan LM, Patel R: Real-time PCR detection of Mycoplasma pneumoniae in respiratory specimens. Diagn Microbiol Infect Dis. 2013 Nov;77[3]:202-205)

Report Available

3 to 4 days

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87581

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MPRP Mycoplasma pneumoniae PCR 29257-3

 

Result ID Test Result Name Result LOINC Value
SRCMP Specimen source 31208-2
62394 Mycoplasma pneumoniae PCR 29257-3

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Laboratory Test Directory Additional Information:

Aliases: Mycoplasma by DNA PCR Sputum (BAL)