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Epic Code LAB844 Protein Electrophoresis, Serum, IFE if Indicated

Test Name Alias

Protein Electrophoresis IFE if Indicated | SPEP | S-PEP | Immunofixation | 8306

Interface Order Alias

10811

Clinical Information

Test will include serum protein and pathologist interpretation. For guidance on ordering protein and urine electrophoresis, please refer to Orders- Protein Orderable Changes. 

Ordering Instructions

Protein Electrophoresis cannot be ordered a second time within a 2 week window in Laboratory Information System (LIS)

 

Collection Instructions

Specimen Collection: Blood

Container(s): Gold Top (Serum Separator-SST Gel)

Preferred Volume to Collect: 5.0 mL

Minimum Volume to Collect: 2.0 mL

Neonate Volume to Collect: 2.0 mL

Capillary collect ok: Yes

Microtainer acceptable: Yes

Collection Instructions:

  • Fasting specimen preferred, but not required.
  • Gently invert gold tube(s) 8 - 10 times to mix clot activator with blood.
  • Specimen should be processed within 2 hours – See Processing Instructions.

 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

 

Processed Specimen: Serum

Centrifuge/Spin: Yes

Aliquot: No

Processing Instructions:

  • Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.

Transport Temperature: Refrigerate

Rejection Criteria

Specimen not centrifuged within 2 hours

Specimen Stability

Ambient: 8 hours

Refrigerate: 7 days

Laboratory Retention: 7 days

Reflex Information

Reflex approved by Medical Executive Committee. Please see link: Reflex Testing Document

Test Frequency

Available Monday – Friday, routine TAT 3 to 5 days.

Reference Range

Protein total

0 up to 8 Days: 4.5 – 7.0 g/dL
8 Days up to 3 Years: 5.5 – 7.5 g/dL
>3 Years: 6.0 – 8.0 g/dL

 

Albumin SPE: 3.7 – 4.9 g/dL

 

Alpha 1 Globulin: 0.2 – 0.4 g/dL

 

Alpha 2 Globulin: 0.5 – 0.9 g/dL

 

Beta Globulin:  0.6 – 1.0 g/dL

 

Gamma Globulin: 0.6 – 1.4 g/dL

 

 

Performing Department

Multiple Departments: Chemistry and Immunochemistry

Performing Department Laboratory Location

Corewell Health Reference Laboratory, Grand Rapids, MI

Methodology

Capillary Zone Electrophoresis

CPT

84165, 84165, 84165, 84165

CDM Code

3018416501, 3018416501, 84165, 84165

Epic Test ID

1230101084

Mayo Access Code

SHO8306

Reviewed Date

3/23/2022