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Test Code LAB844 Protein Electrophoresis, Serum, IFE if Indicated

Test Name Alias

Protein Electrophoresis IFE if Indicated | SPEP | S-PEP | Immunofixation | 8306

Clinical Information

Test will include serum protein and pathologist interpretation.

Collection Instructions

Specimen Collection: Blood

Container(s): Gold Top (Serum Separator-SST Gel)

Preferred Volume to Collect: 5.0 mL

Minimum Volume to Collect: 2.0 mL

Neonate Volume to Collect: 2.0 mL

Capillary collect ok: Yes

Microtainer acceptable: Yes

Collection Instructions:

  • Fasting specimen preferred.
  • Gently invert gold tube(s) 8 - 10 times to mix clot activator with blood.
  • Specimen should be processed within 2 hours – See Processing Instructions.
  • Protein Electrophoresis cannot be ordered a second time within a 2 week window in Laboratory Information System (LIS)

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Processed Specimen: Serum

Centrifuge/Spin: Yes

Aliquot: No

Processing Instructions:

  • Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.

Transport Temperature: Refrigerate

Specimen Transport Temperature


Rejection Criteria

Specimen not centrifuged within 2 hours

Reflex Information

Reflex approved by Medical Executive Committee. Please see link: Reflex Testing Document

Specimen Stability

Ambient: 8 hours

Refrigerate: 7 days

Frozen: aliquot serum for longer storage

Laboratory Retention: 7 days

Test Frequency

Available Monday – Friday, routine TAT 5 days.

Reference Range

Protein total

0 up to 8 Days: 4.5 – 7.0 g/dL
8 Days up to 3 Years: 5.5 – 7.5 g/dL
>3 Years: 6.0 – 8.0 g/dL


Albumin SPE: 3.7 – 4.9 g/dL


Alpha 1 Glob: 0.2 – 0.4 g/dL


Alpha 2 Glob: 0.5 – 0.9 g/dL


Beta Glob:  0.6 – 1.0 g/dL


Gamma Glob: 0.6 – 1.4 g/dL



Performing Department

Multiple Departments: Chemistry and Immunochemistry

Performing Department Laboratory Location

Spectrum Health Regional Laboratory, Grand Rapids, MI


Capillary Zone Electrophoresis



Professional fee for Pathologist Interpretation

CDM Code


Epic Test ID


Interface Order Alias


Mayo Access Code


Reviewed Date