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Epic Code LAB8870 Hepatitis C Virus RNA Quantitation by RT-PCR

Test Name Alias

Hepatitis C VirusRNA Quantitative | 4185

Interface Order Alias

10170

Ordering Instructions

If one or more of the following tests are to be ordered, only one EDTA plasma tube or one serum tube (minimum volume 2 mL) needs to be provided:

  • Hepatitis C Virus RNA Quantitation by RT-PCR
  • Hepatitis C Genotype with Amplification

In addition, positive HCV specimens will be stored by the laboratory for 1 month, so additional follow-up testing can be added to the original order by FAXing the completed “Additional/Add-on Laboratory Request” form to Spectrum Health Lab Central at 616-267-2751.

Collection Instructions

Specimen Collection: Blood

 

Container(s): 6 mL Pink Top (EDTA) OR 5 mL Gold top (Serum Separator-SST Gel)

Preferred Volume to Collect: Full tube (6 mL or 5 mL)

 

Collection Instructions:

  • Gently invert EDTA tube(s) 8 - 10 times after collection.
  • Specimen should be processed within 4 hours – See Processing Instructions.

 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

  • Processed Specimen: Plasma or Serum
  • Centrifuge/Spin: Yes
  • Aliquot: Yes
  • Processes Minimum Volume: 2.0 mL Plasma or Serum
  • Processing Instructions:
    • Plasma is preferred specimen type
    • With room temperature storage, centrifuge whole blood in EDTA or Serum Separator tube within 4 hours of collection. 
    • With refrigerated temperature storage, centrifuge whole blood in EDTA or Serum Separator tube within 72 hours of collection. 
    • Transfer plasma  or serum to a screw-capped transport tube.
    • Plasma or serum needs to be stored frozen in screw-capped transport tube after separation from cells
  • Transport temperature: Prefer that plasma or serum be frozen for transport

Specimen Stability

Ambient: Stability of whole blood in EDTA or a Serum Separator tube is 4 hours

Refrigerate: 3 days

Frozen: 60 days

Laboratory Retention: 1 month

Reflex Information

Reflex approved by MEC

  • Specimens with Positive Hepatitis C Antibody results will be automatically reflexed to the quantitative Hepatitis C Virus RNA test

Test Frequency

Test performed twice weekly, usual TAT 3 - 5 days

Reference Range

HCV

Not Detected: <12 IU/mL

 

Linear range of positive results

Positive: 12 to 100,000,000 (1e8) IU/mL

Equivocal: Detected but below lower limit of quantitation (<12 IU/mL)

Performing Department

MOLECULAR DIAGNOSTICS

Performing Department Laboratory Location

Spectrum Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Quantitative Reverse Transcriptase Real-Time Polymerase Chain Reaction (RT-PCR)

CPT

87522

CDM Code

3900258

Epic Test ID

1230100767

LOINC

HCV RNA Result : 11259-9

HCV RNA Viral Load : 11011-4

Beaker LOINC

HCV RNA RESULT: 11259-9
HCV RNA VIRAL LOAD: 29609-5

Mayo Access Code

SHO4185

Reviewed Date

8/23/2021

Beaker Names

Beaker Procedure Name: HEPATITIS C VIRUS RNA QUANTITATION BY RT PCR
Beaker Display Name: Hepatitis C Virus RNA Quantitation by RT-PCR
BEAKER TEST NAME: HEPATITIS C VIRUS RNA QUANTITATIVE BY RT-PCR
BEAKER TEST REPORT NAME: Hepatitis C Virus RNA Quantitative by RT-PCR

Beaker Synonyms

No synonym on file

Beaker Collection and Specimen Handling

BEAKER COLLECTION: +(4)S/PO/F

Beaker Location, Container and Temperature

LC MOLECULAR DIAGNOSTICS: 6 ML PINK EDTA F (Preferred)
LC MOLECULAR DIAGNOSTICS: GOLD TOP SST F (Acceptable)-
LC MOLECULAR DIAGNOSTICS: GOLD TOP SST TUBE R (Acceptable)