Sign in →

Epic Code LAB9150 Hepatitis C Genotype

Important Note

This testing may be cancelled and reordered as a Reference Miscellaneous [LAB848]. Please see Hepatitis C Virus Genotype by Sequencing for collection and results information.

Test Name Alias

Hepatitis C Genotype w/Amplification | Hep C Genotype | 4434 | HCV

Interface Order Alias

10801

Quick Collect

+S/PO/F

Ordering Instructions

Hepatitis C Virus RNA Quantitation by RT-PCR testing (test code #LAB8870) must be ordered before Hepatitis C Genotyping will be performed.  HCV Genotype testing is intended to be used as a follow-up procedure for patients who have been identified with an active HCV infection, with an HCV viral load adequate for HCV Genotyping (over 500 IU/mL).

 

If one or both of the following tests are to be ordered, only one EDTA tube (minimum volume 6 mL) or one Gold Top SST tube (minimum volume 5 mL) needs to be collected:

 

Hepatitis C Virus RNA Quantitation by RT-PCR

Hepatitis C Genotype with Amplification

 

In addition, positive HCV specimens will be stored by the laboratory for 1 month, so additional follow-up testing can be added to the original order by FAXing the completed “Additional/Add-on Laboratory Request” form to Spectrum Health Lab Central at 616-267-2751.

Collection Instructions

Specimen Collection: Blood

 

Container(s): 6 mL Pink Top (EDTA) OR 5 mL Gold top (Serum Separator-SST Gel)

Preferred Volume to Collect: Full tube (6 mL or 5 mL)

 

Collection Instructions:

  • Gently invert EDTA tube(s) 8 - 10 times after collection.
  • Specimen should be processed within 4 hours – See Processing Instructions.

 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

  • Processed Specimen: Plasma or Serum
  • Centrifuge/Spin: Yes
  • Aliquot: Yes
  • Processes Minimum Volume: 2.0 mL Plasma or Serum
  • Processing Instructions:
    • Plasma is preferred specimen type
    • With room temperature storage, centrifuge whole blood in EDTA or Serum Separator tube within 4 hours of collection. 
    • With refrigerated temperature storage, centrifuge whole blood in EDTA or Serum Separator tube within 72 hours of collection. 
    • Transfer plasma or serum to a screw-capped transport tube.
    • Plasma or serum needs to be stored frozen in screw-capped transport tube after separation from cells
  • Transport temperature: Prefer that plasma or serum be frozen for transport

Specimen Stability

Ambient: 24 hours

Refrigerate: 3 days

Frozen: 60 days

Laboratory Retention: 1 month

Reflex Information

Reflex approved by MEC

Test Frequency

Test performed once per week, usual TAT less than 7 days.

Reference Range

Genotypes 1a, 1b, 2a/c, 2b, 3, 4, 5 and 6 reported

Performing Department

Molecular Diagnostics

Performing Department Laboratory Location

Corewell Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Multiplex Reverse Transcription - Polymerase Chain Reaction (RT-PCR) followed by solid phase electrochemical analysis.

CPT

87902, 87521

CDM Code

3068790201, 3068752101

Epic Test ID

1230100766

LOINC

HCV Genotype Comment: 8251-1

HCV Genotyping: 32286-7

HCV Qual RT-PCR: 11259-9

Mayo Access Code

SHO4434

Reviewed Date

6/22/2023